Table 1.
Results of non-linear regression analysis of ELISA-measured plasma PfHRP2 concentration vs. digital reading, and of interobserver variability assessment for each of four kits
| Core™ | Paracheck® | First Response® | SD Bioline | |
|---|---|---|---|---|
| Digital assessment | ||||
| Top (reading units) | 1867 | 1796 | 807.2 | 1414 |
| HillSlope | 1.408 | 1.904 | 7.058 | 1.453 |
| EC50 (ng/ml) | 63.14 | 36.25 | 75.91 | 66.99 |
| EC50 (95 % CI) | 48.38–82.40 | 31.69–41.48 | 49.63–116.1 | 47.35–94.80 |
| Goodness of fit (r2) | 0.9082 | 0.9627 | 0.5661 | 0.8516 |
| Visual assessment kappa-statistic | ||||
| 0—no visible band | 0.7931 | 1 | 0.7785 | 0.7857 |
| 1—faint line | 0.3197 | 1 | 0.3262 | 0.3056 |
| 2—faint band | 0.7869 | 0.7432 | 0.6019 | 0.6296 |
| 3—clear band weaker than control | 0.7778 | 0.9187 | 0.5879 | 0.6384 |
| 4—band at least as strong as control | 1 | 0.9664 | 0.5662 | 0.85 |
| Combined | 0.8602 | 0.9377 | 0.5865 | 0.7198 |