Table 5. Chicken carcase swab samples tested with mPCR-HRM.
Mean±SD of the melting points and GCP produced by isolates when ATCC29428 or ATCC33559 were used as reference strains.
| Culture | mPCR-HRM | |||||||
|---|---|---|---|---|---|---|---|---|
| Melting points | Mean GCP±SD (%) | |||||||
| Isolate ID. | culture | species | Peak 1 (°C) | Peak 2 (°C) | Peak 3 (°C) | ATCC33559 used as reference strain | ATCC29428 used as reference strain | Genotype |
| ATCC33559 | Positive | C. coli | 76.1±0.1 | 82.1±0.1 | 99.9±0.1 | 7.7±0.1 | C. coli | |
| ATCC29428 | Positive | C. jejuni | 78.5±0.0 | 81.8±0.0 | 7.7±0.3 | 99.9±0.1 | C. jejuni | |
| 212250 | Negative | NA a | 76.2±0.1 | 81.9±0.3 | 88.0±0.5 | 17.5±0.2 | C. coli | |
| 212251 | Negative | NA | 76.4±0.1 | 81.9±0.2 | 90.3±0.3 | 16.3±0.2 | C. coli | |
| 212252 | Negative | NA | 77.5±1.3 | 80.2±2.4 | 81.9±0.1 | 32.4±1.1 | 49.1±1.1 | Variation |
| 212253 | Negative | NA | 78.5±0.1 | 81.6±0.1 | 4.6±0.4 | 96.3±1.4 | C. jejuni | |
| 212254 | Negative | NA | 76.4±0.0 | 81.9±0.0 | 92.2±0.2 | 11.9±0.2 | C. coli | |
| 212255 | Negative | NA | 76.0±0.1 | 78.3±0.1 | 82.0±0.0 | 49.3±2.1 | 50.3±2.5 | Variation |
| 212256 | Negative | NA | 78.8±0.1 | 81.2±0.1 | 2.5±0.2 | 71.1±0.9 | C. jejuni | |
| 212257 | Negative | NA | 76.3±0.1 | 81.8±0.1 | 81.7±3.2 | 11.9±0.4 | C. coli | |
| 212258 | Negative | NA | 78.8±0.1 | 80.9±0.0 | 1.9±0.2 | 58.3±2.5 | C. jejuni | |
| 212259 | Negative | NA | 76.2±0.1 | 82.1±0.1 | 95.9±2.5 | 9.0±0.5 | C. coli | |
| 212262 | Negative | NA | NA | NA | NA | NA | NA | Negative |
| 212263 | Negative | NA | 78.8±0.0 | 81.5±0.0 | 5.4±0.2 | 71.1±1.8 | C. jejuni | |
| 212264 | Negative | NA | 78.9±0.1 | 81.4±0.0 | 4.4±0.1 | 58.8±0.5 | C. jejuni | |
| 212265 | Negative | NA | 78.3±0.0 | 81.5±0.0 | 6.8±0.2 | 92.2±0.7 | C. jejuni | |
| 212266 | Negative | NA | NA | NA | NA | NA | NA | Negative |
| 212514 | Positive | C. jejuni | 78.5±0.0 | 81.9±0.1 | 5.5±0.1 | 91.8±0.6 | C. jejuni | |
| 212515 | Positive | C. jejuni | 78.5±0.0 | 81.6±0.0 | 5.4±0.2 | 94.5±0.5 | C. jejuni | |
| 212516 | Negative | NA | NA | NA | NA | NA | NA | Negative |
| 212517 | Positive | C. jejuni | 78.5±0.0 | 81.8±0.0 | 3.8±0.3 | 92.4±1.4 | C. jejuni | |
| 212518 | Positive | C. jejuni | 78.5±0.0 | 81.8±0.0 | 7.6±0.1 | 90.3±2.0 | C. jejuni | |
| 212519 | Positive | C. jejuni | 78.5±0.0 | 81.7±0.1 | 4.9±0.2 | 94.4±0.4 | C. jejuni | |
| 212520 | Positive | C. jejuni | 78.5±0.0 | 81.7±0.1 | 6.6±0.1 | 91.9±1.8 | C. jejuni | |
| 212521 | Positive | C. jejuni | 78.5±0.0 | 81.5±0.1 | 4.8±0.5 | 94.4±0.8 | C. jejuni | |
| 212522 | Positive | C. jejuni | 78.7±0.0 | 80.9±0.1 | 5.9±0.7 | 89.8±4.1 | C. jejuni | |
| 212523 | Negative | NA | 78.7±0.0 | 80.8±0.0 | 0.9±0.2 | 56.8±0.8 | C. jejuni | |
aNA, Not applicable