Table 1. The respiratory chain and the phycobilisomes affect chl a parameters.
chl a | F0 | F0' | FM | FV | ϕPSII max | ϕPSII GL | |
---|---|---|---|---|---|---|---|
WT | 2.95 ± 0.025 | 601 ± 2 | 585 ± 1 | 1098 ± 17 | 474 ± 15 | 0.45 ± 0.006 | 0.37 ± 0.004 |
M55 | 1.63 ± 0.005 | 471 ± 18 | 402 ± 14 | 740 ± 45 | 357 ± 12 | 0.36 ± 0.015 | 0.27 ± 0.014 |
ΔOx | 2.71 ± 0.046 | 494 ± 9 | 494 ± 3 | 883 ± 2* | 356 ± 10* | 0.44 ± 0.008* | 0.36 ± 0.014 |
PAL | 3.18 ± 0.009 | 49 ± 1 | 46 ± 1 | 129 ± 3* | 80 ± 3* | 0.62 ± 0.014* | 0.46 ± 0.016 |
Chlorella | 3.61 ± 0.093 | 255 ± 20 | 277 ± 19 | 1045 ± 66 | 780 ± 47 | 0.76 ± 0.004 | 0.49 ± 0.008 |
Values shown were derived from the experiments as depicted and described in Fig 1. All values were normalized to OD730. chl a, chlorophyll a concentration in mg L-1; F0, level of fluorescence in the dark; FM, fluorescence measured by applying a strong light pulse (2,000 μmol photons m-2 s-1) in the dark; FV, variable fluorescence (FM-F0); ϕPSII, quantum yield of PSII ((FM-F0)/FM) calculated using F0 and FM (max) or F0' and FM' under growth light conditions (GL).
*, derived using FM values after addition of 20 μM DCMU in the light (400 μmol photons m-2 s-1). Values are averages of duplicate measurements with standard deviation.