Fig 5. CRKN infection of a double-susceptible white clover genotype of cv. Huia influences the transcript abundance of ET biosynthetic and JA signalling genes.

(A) Changes in transcript abundance of Tr-ACS1, Tr-ACO2, Tr-ACO3 and Tr-COI1, as indicated, in roots excised from control or CRKN-infected plants, as indicated, at the times indicated. (B) Changes in transcript abundance of Tr-ACS1, Tr-ACO2, Tr-ACO3 and Tr-COI1, as indicated, in the first fully expanded (FFE) leaf tissue excised from control plants or plants with CRKN-infected roots, as indicated, at the times indicated. Bars represent mean values, ± SEM, of three biological replicates (n = 3). The control for each data point was set to 1. The letters a and b indicate statistically significant differential expression at 4 dpi and the letters c and d indicate statistically significant differential expression at 8 dpi when compared with the respective control plants that were not challenged with CRKN eggs; the same letter above the control and treatment bars indicate insignificant difference.