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. 2015 Sep 22;11(9):e1005514. doi: 10.1371/journal.pgen.1005514

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© 2015 Liu, Grant

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) The membrane-to-cytosol ratio of RAB-5 increased in tbc-2, rab-10 and amph-1 mutants. Post-nuclear worm lysates were subject to centrifugation at 100,000g for 1 hour. 100P corresponds to the pellets and 100S represents the supernatants after the 100,000g centrifugation. (B) Loading control with actin antibody. (C) Quantification of the membrane-to-cytosol ratio (100P/100S) of RAB-5 in appropriate genetic backgrounds. The ratio of membrane-bound VS cytosolic RAB-5 was determined by densitometry. The standard deviations from three independent experiments are shown. **P<0.01 (student's t test).