Figure 7. Chronic neonatal hypoxia modulates lung DNA methylation in IGF-1 promoter region.
(A) Location of CpG sites along proximal mouse IGF-1 promoter region. (B) Genomic DNA isolated from normoxic and hypoxic lungs was analyzed for cytosine methylation at multiple sites along the mIGF-1 promoter region in the lungs. * p<0.05 compared to normoxia control. (C) HDAC inhibition with apicidin normalizes hypoxia-induced elevation of cytosine methylation at CpG1 site (position 87857993) along pulmonary IGF-1 promoter region (Chr10). * p<0.05 compared to normoxia controls. # p<0.05 compared to hypoxia vehicle controls.