Figure 4.

NVI mice display cross-sensitization in both hind paw and colon. (A) The withdrawal threshold of the hind paw to a mechanical stimulus was significantly lower in NVI mice (n = 8) compared with naive mice (n = 7). (B) NVI mice (n = 8) displayed a significantly shorter withdrawal latency to thermal stimulation of the hind paw than naive mice (n = 7). (C) NVI mice (n = 5) also displayed significantly higher VMR during CRD than naive mice (n = 5) over the entire dissension series (P < 0.05), as well as at the highest intraballoon pressure applied. (D) Representative Western blots are shown for TRPA1, TRPV1, CRF₁ and corresponding GAPDH protein expression with bands at 127, 85, 55, and 35 kD, respectively, in the colon of naive and NVI mice. Only TRPV1 protein expression in the colon was affected by NVI (n = 4), with a significant decrease compared with naive (n = 6). (A, B, and D): *, ****P < 0.05, 0.0001 vs naive; Student t test. (C): *P < 0.05 vs naive; 2-way repeated-measures ANOVA and Bonferroni's posttest.