. Author manuscript; available in PMC: 2016 Sep 1.

Published in final edited form as: Eur J Neurosci. 2015 Jul 23;42(6):2271–2282. doi: 10.1111/ejn.12996

Table 1.

Experimental design

Group	Mouse	Vector(s)	C1 cells	R N cells	Breathing stimulation
1	DβH^+/+;;Vglut2^+/+ (C57)	PRSx8-Cre LV + DIO-ChR2-eYFP AAV	ChR2+/Vglut2+	ChR2+/Vglut2+	Yes⁺
2	DβH^Cre/0;;Vglut2^fl/fl	PRSx8-ChR2-eYFP LV	ChR2+/Vglut2−	ChR2+/Vglut2+	Yes⁺
3	DβH^+/+;;Vglut2^fl/fl	PRSx8-Cre LV + DIO-ChR2-eYFP AAV	ChR2+/Vglut2−	ChR2+/Vglut2−	No⁺

Groups 1-3: new experiments performed in the present study.

⁺

expected result if selective stimulation of RTN neurons increases breathing by releasing glutamate.