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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Stem Cells. 2015 Aug 11;33(10):2985–2994. doi: 10.1002/stem.2109

Figure 3.

Figure 3

Co-culture of HER2Ab-NSCs with BT474Br cells. BT474Br cells were stained with epithelia specific keratins (Pseudo colour Green) to differentiate NSC (Kertatin negative cells). HER2Ab was visualized using anti-Human Alexa Fluor®-647antibody (Far Red). Top panel demonstrates the production of antibody (Red) by HER2Ab-NSCs and cell membrane staining of breast cancer cells with anti-HER2Ab (Red) (A-E). Yellow arrow head indicates breast cancer cells stained with keratin, whereas white arrows indicate HER2Ab-NSCs. (D) Composite image of NSCs with breast cancer cells, (E) 3D surface rendition of the Z stack composite image, inset shows enlarged views of white boxes of D and E indicating cytoplasmic staining in HER2Ab-NSCs. Vector control NSCs showed absence of anti-HER2Ab signal (F-I). The experiments were repeated two times. Scale bar = 10μM