Table 2.
Performance of the Method: CT DNA Modified with NM (1μM) With-day and Between-day Reproducibility.a,b
| Adduct/107 bases | Day 1 (pH7) | Day 2 (pH7) | Day 3 (pH7) | CV (%) within-day | CV (%) between-day | |
|---|---|---|---|---|---|---|
|
| ||||||
| NM-G | Mean | 20.7 | 19.4 | 19.9 | ||
| SD | 1.7 | 0.2 | 1.3 | |||
| RSD (%) | 8.3 | 1.1 | 6.7 | 6.4 | 6.2 | |
|
| ||||||
| G-NM-G | Mean | 132 | 118 | 127 | ||
| SD | 14.1 | 1.3 | 9.7 | |||
| RSD (%) | 10.6 | 1.1 | 7.5 | 8.2 | 9.1 | |
|
| ||||||
| NM-FapyG | Mean | 19.9 | 18.8 | 19.2 | ||
| SD | 1.1 | 0.2 | 1.1 | |||
| RSD (%) | 5.7 | 1.0 | 5.4 | 4.8 | 5.2 | |
|
| ||||||
| FapyG-NM-G | Mean | 3.8 | 3.7 | 3.7 | ||
| SD | 0.2 | 0.1 | 0.2 | |||
| RSD (%) | 6.7 | 1.3 | 7.4 | 5.9 | 5.5 | |
a
CT DNA (50 μg/mL in potassium phosphate (50 mM, pH 7.0) was subjected to neutral thermal hydrolysis at 95 °C for 4 h.
b
FapyG-NM-FapyG was below the LOQ value (0.3 adduct per 107 bases) and not reported.