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. 2015 Sep 22;10(9):e0137845. doi: 10.1371/journal.pone.0137845

Fig 5. Down-regulation of miRNA-10b by BCL2 inhibitors is associated with the inhibition of proliferation by induction of apoptosis.

Fig 5

Medulloblastoma cells were incubated with BCL2 inhibitors ABT-737 and ABT-199 for 48 hours. DMSO 0.1% was used as a control treatment. Total RNA was extracted from DAOY and UW228 cells, and subjected to RT-PCR for the analysis of miRNA-10b expression under the influence of BCL2 inhibitors (A) ABT-737 and (B) ABT-199. (C) ABT-737 and ABT-199 significantly inhibit the proliferation of medulloblastoma cells measured by MTT assay (D) ABT-737 and ABT-199 significantly induce the apoptosis of medulloblastoma cells measured by cell death detection ELISA. (** P ≤ 0.05 and *** P ≤ 0.001 according to student’s t-test)