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. 2015 Sep 22;10(9):e0137716. doi: 10.1371/journal.pone.0137716

Fig 3. Metabolic dysfunction of cardiomyocytes is dependent on the presence of rVλ6Wil fibrils.

Fig 3

(A) Supernatants from a suspension of rVλ6Wil fibrils generated by centrifugation of a fibril suspension at either 435,000 x g or 16,000 x g do not decrease MTT reduction by cardiomyocytes (supernatants were used at a 1x or 10x volume equivalent [vol. eq] to the unfractionated fibril suspension). Filtrate from a suspension of rVλ6Wil fibrils passed through a 100,000 (100K) MWCO filter did not contain material that affected MTT reduction (n = 3, per assay). (B) HPLC analysis of supernatant samples from the 435,000 x g (435K) and 16, 000 x g (16K) centrifugation as compared to the same volume of starting rVλ6Wil monomer (M) material. (C) Over the course of rVλ6Wil fibrillogenesis, only samples of the reaction mixture containing ThT-positive material significantly decreased MTT reduction when added for 24 h to AC10 cardiomyocytes. Gray circles, thioflavin T fluorescence (excitation = 450 nm; emission = 490 nm); black circles, MTT reduction (n = 3 sample per time point).