Table 2. Distribution of amphotericin B MICs of 136 clinical isolates of Aspergillus species, determined by three methods.
| Species complex | Species (N of isolates) | Test method* | N of isolates for which the AMB MIC (µg/mL) was:† | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.03 | 0.06 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | |||
| A. fumigatus complex | A. fumigatus (56) | CLSI | 1 | 22 | 28 | 5 | |||||||
| Etest-MH | 1 | 1 | 3 | 6 | 26 | 16 | 3 | ||||||
| Etest-RPG | 2 | 1 | 3 | 18 | 18 | 12 | 2 | ||||||
| A. lentulus (2) | CLSI | 2 | |||||||||||
| Etest-MH | 2 | ||||||||||||
| Etest-RPG | 2 | ||||||||||||
| Total (58) | CLSI | 1 | 22 | 28 | 5 | 2 | |||||||
| Etest-MH | 1 | 1 | 3 | 6 | 26 | 16 | 3 | 2 | |||||
| Etest-RPG | 2 | 1 | 3 | 18 | 18 | 12 | 2 | 2 | |||||
| A. niger complex | A. niger (7) | CLSI | 5 | 2 | |||||||||
| Etest-MH | 1 | 2 | 3 | 1 | |||||||||
| Etest-RPG | 1 | 1 | 2 | 3 | |||||||||
| A. awamori (15) | CLSI | 7 | 5 | 3 | |||||||||
| Etest-MH | 1 | 1 | 7 | 3 | 3 | ||||||||
| Etest-RPG | 1 | 3 | 9 | 2 | |||||||||
| A. tubingensis (10) | CLSI | 1 | 1 | 8 | |||||||||
| Etest-MH | 3 | 4 | 2 | 1 | |||||||||
| Etest-RPG | 2 | 3 | 4 | 1 | |||||||||
| Total (32) | CLSI | 1 | 1 | 15 | 10 | 5 | |||||||
| Etest-MH | 4 | 5 | 10 | 5 | 7 | 1 | |||||||
| Etest-RPG | 1 | 3 | 7 | 15 | 5 | 1 | |||||||
| A. flavus complex | A. flavus/A. oryzae (22) | CLSI | 7 | 13 | 2 | ||||||||
| Etest-MH | 2 | 1 | 2 | 2 | 6 | 2 | 7 | ||||||
| Etest-RPG | 1 | 4 | 8 | 8 | 1 | ||||||||
| A. tamarii (2) | CLSI | 1 | 1 | ||||||||||
| Etest-MH | 1 | 1 | |||||||||||
| Etest-RPG | 2 | ||||||||||||
| Total (24) | CLSI | 1 | 8 | 13 | 2 | ||||||||
| Etest-MH | 3 | 1 | 3 | 2 | 6 | 2 | 7 | ||||||
| Etest-RPG | 2 | 1 | 4 | 8 | 8 | 1 | |||||||
| A. terreus complex | A. terreus (15) | CLSI | 1 | 4 | 6 | 4 | |||||||
| Etest-MH | 1 | 2 | 3 | 3 | 2 | 3 | 1 | ||||||
| Etest-RPG | 1 | 5 | 5 | 1 | 1 | 1 | 1 | ||||||
| A. versicolor complex | A. sydowii (5) | CLSI | 2 | 3 | |||||||||
| Etest-MH | 1 | 1 | 2 | 1 | |||||||||
| Etest-RPG | 1 | 2 | 2 | ||||||||||
| A. versicolor (1) | CLSI | 1 | |||||||||||
| Etest-MH | 1 | ||||||||||||
| Etest-RPG | 1 | ||||||||||||
| Total (6) | CLSI | 3 | 3 | ||||||||||
| Etest-MH | 1 | 1 | 2 | 2 | |||||||||
| Etest-RPG | 1 | 2 | 3 | ||||||||||
| A. ustus complex | A. calidoustus (1) | CLSI | 1 | ||||||||||
| Etest-MH | 1 | ||||||||||||
| Etest-RPG | 1 | ||||||||||||
| Total | All (136) | CLSI | 1 | 1 | 17 | 33 | 48 | 27 | 9 | ||||
| Etest-MH | 1 | 1 | 11 | 15 | 43 | 28 | 21 | 6 | 8 | 2 | |||
| Etest-RPG | 3 | 1 | 3 | 14 | 40 | 32 | 18 | 12 | 1 | 8 | 4 | ||
*CLSI, the CLSI M38-A2 broth microdilution method; Etest-MH, Etest on Mueller-Hinton agar supplemented with glucose and methylene blue; Etest-RPG, Etest on RPMI agar supplemented with glucose; †Etest MICs were rounded up to the next highest twofold dilution value.
Abbreviations: AMB, Amphotericin B; MIC, minimum inhibitory concentration; Etest-MH, Etests using Mueller-Hinton agar; Etest-RPG, Etests using RPMI agar.