Table 2.
Mean quantitative metAFLP characteristics for different genotype sets and in vitro tissue culture regeneration methods
| MetAFLP quantitative characteristics | Si | RA (%) | RM (%) | RE (%) | Mean (all regeneration approaches) (%) |
|---|---|---|---|---|---|
| Sequence variation (SV) | S1 | 11.02 ± 0.93 | 11.62 ± 1.62 | 11.49 ± 0.62 | 11.38 ± 1.18 |
| S2 | 19.73 ± 1.14 | 18.55 ± 1 | 19.88 ± 0.75 | 19.64 ± 1.04 | |
| S3 | 26.37 ± 0.76 | 25.76 ± 0.82 | 21.45 ± 0.82 | 24.54 ± 2.26 | |
| S4 | 20.87 ± 0.9 | 20.67 ± 0.92 | 21.56 ± 0.74 | 21.10 ± 0.9 | |
| Mean (all sets) | 19.5 ± 5.51 | 19.15 ± 5.19 | 18.6 ± 4.35 | 19.16 ± 5.21 | |
| Demethylation (DMV) | S1 | 4.5 ± 0.54 | 4.07 ± 0.6 | 4.88 ± 0.32 | 4.48 ± 0.6 |
| S2 | 5.67 ± 0.75 | 6.28 ± 0.3 | 6.22 ± 0.57 | 5.94 ± 0.64 | |
| S3 | 5.34 ± 0.36 | 5.35 ± 0.47 | 5.74 ± 0.36 | 5.48 ± 0.42 | |
| S4 | 6.12 ± 0.43 | 5.86 ± 0.66 | 6.12 ± 0.49 | 6.04 ± 0.53 | |
| Mean (all sets) | 5.4 ± 0.75 | 5.39 ± 0.99 | 5.74 ± 0.7 | 5.48 ± 0.86 | |
| De novo methylation (DNMV) | S1 | 2.84 ± 1.14 | 3.58 ± 0.99 | 3.04 ± 0.79 | 3.18 ± 1.02 |
| S2 | 5.48 ± 1.61 | 4.08 ± 1.1 | 4.63 ± 0.85 | 5.0 ± 1.35 | |
| S3 | 4.85 ± 1.02 | 5.06 ± 0.78 | 4.35 ± 0.61 | 4.71 ± 0.87 | |
| S4 | 5.29 ± 0.74 | 5.21 ± 1.05 | 4.71 ± 0.75 | 5.03 ± 0.86 | |
| Mean (all sets) | 4.61 ± 1.59 | 4.48 ± 1.16 | 4.18 ± 1.02 | 4.48 ± 1.29 | |
| Total tissue culture-induced variation (TTCIV) | S1 | 18.36 ± 1.43 | 19.28 ± 1.8 | 19.41 ± 0.91 | 19.02 ± 1.48 |
| S2 | 30.88 ± 0.89 | 28.92 ± 0.9 | 30.73 ± 0.84 | 30.59 ± 1.1 | |
| S3 | 36.55 ± 1.14 | 36.17 ± 0.83 | 31.55 ± 0.93 | 34.73 ± 2.36 | |
| S4 | 32.28 ± 1.1 | 31.74 ± 1.25 | 32.39 ± 1 | 32.17 ± 1.11 | |
| Mean (all sets) | 29.51 ± 6.84 | 29.02 ± 6.46 | 28.52 ± 5.55 | 29.12 ± 6.5 | |
| Genome methylation (GM) | S1 | 7.95 ± 1.84 | 9.21 ± 1.34 | 7.07 ± 1.11 | 8.14 ± 1.74 |
| S2 | 16.33 ± 2.03 | 14.80 ± 1.5 | 15.17 ± 1.83 | 15.73 ± 1.83 | |
| S3 | 10.71 ± 1.39 | 10.88 ± 0.86 | 10.54 ± 0.89 | 10.68 ± 1.13 | |
| S4 | 14.45 ± 1.09 | 14.89 ± 1.43 | 13.63 ± 1.01 | 14.24 ± 1.24 | |
| Mean (all sets) | 12.36 ± 2.58 | 12.44 ± 1.96 | 11.6 ± 2.55 | 12.19 ± 2.4 | |
| Sites affected by methylation (SAM) | S1 | 7.34 ± 1.07 | 7.66 ± 1.18 | 7.92 ± 0.85 | 7.64 ± 1.06 |
| S2 | 13.66 ± 1.3 | 12.56 ± 0.8 | 13.52 ± 0.75 | 13.48 ± 1.08 | |
| S3 | 10.19 ± 0.99 | 10.41 ± 0.78 | 10.09 ± 0.87 | 10.19 ± 0.96 | |
| S4 | 11.41 ± 0.76 | 11.07 ± 0.99 | 10.83 ± 0.87 | 11.07 ± 0.91 | |
| Mean (all sets) | 10.65 ± 2.54 | 10.42 ± 2.27 | 10.59 ± 2.12 | 10.59 ± 2.36 | |
| Sites with methylated status in donor and regenerant (SMS) | S1 | 3.94 ± 0.68 | 4.29 ± 0.67 | 2.97 ± 0.32 | 3.76 ± 0.8 |
| S2 | 4.42 ± 0.75 | 4.60 ± 0.6 | 4.03 ± 0.43 | 4.23 ± 0.6 | |
| S3 | 3.13 ± 0.42 | 3.13 ± 0.43 | 3.94 ± 0.49 | 3.42 ± 0.57 | |
| S4 | 5.93 ± 0.45 | 6.26 ± 0.55 | 5.71 ± 0.4 | 5.94 ± 0.5 | |
| Mean (all sets) | 4.35 ± 1.03 | 4.57 ± 1.09 | 4.16 ± 1.02 | 4.33 ± 1.08 | |
| Sites with non-methylated status in donor and regenerant (SNMS) | S1 | 70.8 ± 1.05 | 69.71 ± 1.29 | 70.56 ± 0.85 | 70.32 ± 1.17 |
| S2 | 58.65 ± 1.6 | 60.40 ± 0.6 | 59.46 ± 1.01 | 59.15 ± 1.43 | |
| S3 | 56.57 ± 0.92 | 56.92 ± 0.85 | 60.19 ± 0.53 | 57.91 ± 1.72 | |
| S4 | 56.88 ± 0.86 | 56.78 ± 1.04 | 56.93 ± 0.73 | 56.87 ± 0.85 | |
| Mean (all sets) | 60.72 ± 5.89 | 60.95 ± 5.54 | 61.78 ± 5.37 | 61.06 ± 5.7 |
Percentages of sites affected are shown. RA, anther culture-derived regenerants; RM, shed microspore-derived regenerants; RE, immature zygotic embryo-derived regenerants. Si (where i = 1–4) indicates genotype sets encompassing donor plants from four genotypes of DH of triticale cv. Bogo and their regenerants. Standard deviation is shown (±)