Figure 1. Depletion of Pax7+ SCs in P7DTA skeletal muscles.
(A) Scheme demonstrating time of Tmx treatment, Sciatic nerve transection (SNT) surgery, and harvest of tissue. Representative images of TA transverse sections, stained with anti-Pax7 (red), anti-Laminin (white) and DAPI (blue). Red arrowheads indicate Pax7+ cells. (B) Quantification of Pax7+ satellite cell (SC) number from Ctrl and P7DTA TA muscles 6 weeks after sham or SNT surgery. N = 3 mice, 3 sections/mouse, 6 fields/ section. Scale bar = 50 μm. *p < 0.05 compared to Ctrl, **p < 0.05 compared to Ctrl sham, ANOVA/Bonferroni multiple comparisons test.