Figure 4. SC depletion aggravates myofiber type transitions connected to neuromuscular disruption.
(A) Representative images of Ctrl and P7DTA inner TA/EDL muscle regions 6 weeks after sham and SNT surgery stained as indicated with anti-MyHC IIX, all MyHCs except IIX, MyHC IIA, MyHC IIB and MyHC I. Also depicted in Merge IIX+/IIX-, MyHC IIB/IIA and MyHC I labeled images are stains for anti-Laminin (white) and DAPI (blue). (B) Quantification of type IIX pure (green only) and hybrid (green and red, labeled with asterisks) myofiber percentages. (C–E) Quantification of (C) Type IIB (D) Type IIA and (E) Type I fiber percentage. N = 4 mice, 3 sections/mouse, 3 fields/section. Scale bar = 50 μm. *p < 0.05 compared to Ctrl-sham and P7DTA-sham, **p < 0.05 compared to Ctrl sham, P7DTA-sham and Ctrl-SNT, ANOVA/Bonferroni multiple comparisons test.