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. 2015 Oct;22(10):509–513. doi: 10.1101/lm.038810.115

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© 2015 Keistler et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Schematic representation of lesions and disconnection strategy. Coronal sections depicting the largest (dark gray) and smallest (light gray) extent of each lesion for the animals included in behavioral analyses. Sections were collected at 30 µm and immunostained for NeuN (Millipore, Rb 1:500) and glial fibrillary acid protein (GFAP; Dakocytomation, Ms 1:1000) to determine lesion placement and size. (A) Bilateral lesions of ilPFC in both hemispheres. (B) Ipsilateral lesions of ilPFC and NAcS in the same hemisphere, leaving the opposite hemisphere intact. (C) Contralateral lesions of ilPFC and NAcS in opposite hemispheres, functionally disconnecting the two structures.