Table 1.
Details of Experiments 1–6 conducted to assess the temporal characteristics of E2 and VOR effects
| Experiment | Testing order | Group | n | Treatments | Doses | Test latency (min) | Statistics |
||
|---|---|---|---|---|---|---|---|---|---|
| Treatment | Group | Interaction | |||||||
| 1 | 17 | 3 | 13 | PG–VOR | VOR: 50 μg in 1 μl | 30, 120, 240, 480 | F(4,32) = 6.265, p < 0.001 | F(4,8) = 1.008, p = 0.457 | F(4,16) = 2.491, p = 0.014 |
| 2 | 21 | 3 | 12 | PG–VOR–VOR + E2 | VOR: 50 μg in 1 μl; E2: 50 μg in 1 μl | 30, 5 | F(2,18) = 27.946, p < 0.001 | F(2,9) = 1.842, p = 0.214 | F(4,18) = 0.703, p = 0.600 |
| 3 | 19 | 3 | 12 | PG–VOR–VOR + E2 | VOR: 50 μg in 1 μl; E2: 50 μg in 1 μl | 30, 15 | F(2,18) = 28.032, p < 0.001 | F(2,9) = 0.586, p = 0.576 | F(4,18) = 0.314, p = 0.865 |
| 4 | 18 | 3 | 12 | PG–VOR–VOR + E2 | VOR: 50 μg in 1 μl; E2: 50 μg in 1 μl | 30, 120 | F(2,18) = 45.403, p < 0.001 | F(2,9) = 0.606, p = 0.567 | F(4,18) = 1.520, p = 0.239 |
| 5 | 20 | 3 | 12 | PG–VOR–VOR + E2 | VOR: 50 μg in 1 μl; E2: 50 μg in 1 μl | 30, 240 | F(2,18) = 40.876, p < 0.001 | F(2,9) = 1.352, p = 0.306 | F(4,18) = 0.329, p = 0.855 |
| 6 | 4 | 1 | 7 | PG–E2 | E2: 50 μg in 1 μl | 15 | F(1,5) = 1.769, p = 0.241 | F(1,5) = 1.210, p = 0.321 | F(1,5) = 0.236, p = 0.647 |
The experiment numbers (Experiment) follow the order in which the results are described in the manuscript. The actual order in which the experiments were performed is provided in the second column (Testing order). The third column indicates to which group the animals involved in this experiment belonged (see Materials and Methods, General approach). The fourth column provides the number of birds tested in this experiment, while the fifth and sixth columns present the different treatments compared (separated by dashes) as well as the doses and volumes of drugs injected. The latency between injection and test is represented under Test latency. The columns under Statistics provide the results of the statistical analyses.