Figure 8.
Ret ubiquitination is increased in the brains of Gfrα1TM/TM mice. A, The whole brains of E15.5 Gfrα1+/+, Gfrα1TM/+, and Gfrα1TM/TM mice were detergent extracted, and Ret was immunoprecipitated from them. The extent of ubiquitination and Ret protein levels were ascertained by poly-ubiquitin and Ret immunoblotting, respectively. The level of Ret autophosphorylation was determined by phosphotyrosine immunoblotting of the immunoprecipitates, and actin immunoblotting of the supernatants served as a loading control. These experiments were quantified and graphed as the mean ± SEM for Ret ubiquitination (B), Ret protein levels (C), and Ret autophosphorylation (D). *p < 0.05. These experiments were conducted on three to five mice of each genotype from three separate litters.