Table 1.
Percentage of unaligned chromosomes at old centrosomes
| Condition | N0 of repeats | N0 of cells | N0 of chromosomes | % Chromosomes at old centrosomes | 2-tailed Binomial test p |
|---|---|---|---|---|---|
| HeLa-eGFP-centrin1 DMSO | 3 | 33 | 93 | 61.23 | 0.037 |
| hTert-RPE1 10 ng/ml nocodazole | 3 | 161 | 227 | 67.80 | 8.08e-8 |
| hTert-RPE1-eGFP-centrin1 10 ng/ml nocodazole | 7 | 127 | 295 | 71.81 | 1.0e-12 |
| HeLa-eGFP-centrin1 10 ng/ml nocodazole | 3 | 57 | 532 | 63.91 | 1.42e-10 |
| hTert-RPE1 Eg5 inhibition recovery | 3 | 53 | 156 | 74.36 | 8.9e-10 |
| hTert-RPE1 nocodazole recovery | 3 | 59 | 164 | 61.58 | 0.00373 |
| HeLa-eGFP-CENP-A/eGFP-centrin1 10 ng/ml nocodazole | 3 | 142 | 946 | 58.03 | 8.68e-07 |
| HeLa-eGFP-CENP-A/eGFP-centrin1 Eg5 inhibition recovery | 5 | 68 | 306 | 61.11 | 0.000121 |
| hTert-RPE1-eGFP-centrin1 siCtrl 10 ng/ml nocodazole | 3 | 92 | 206 | 68.45 | 1.26e-07 |
| hTert-RPE1-eGFP-centrin1 siNinein 10 ng/ml nocodazole | 4 | 77 | 169 | 66.86 | 0.0000138 |
| hTert-RPE1-eGFP-centrin1 CENP-E inhibitor | 3 | 65 | 393 | 49.87 | n.s* |
| hTert-RPE1-eGFP-centrin1 CENP-E inhibitor 10 ng/ml nocodazole | 3 | 59 | 459 | 52.29 | n.s* |
| hTert RPE-eGFP-centrin1 5 nM Taxol | 3 | 50 | 105 | 43.81 | n.s* |
| hTert RPE-eGFP-centrin1 siDsn1 10 ng/ml nocodazole | 3 | 42 | 162 | 43.82 | n.s* |
| hTert RPE-eGFP-centrin1 siNnf1 10 ng/ml nocodazole | 5 | 21 | 46 | 52.17 | n.s* |
*
Non-significant.