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. 2015 Jan 27;2:2. doi: 10.1186/s40575-015-0013-x

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© Waldo and Diaz; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Genotype determination. A. Three dogs of known genotype were selected. DNA from these dogs was analyzed by qPCR and RCN values calculated. Each bar represents a different set of comparison of PCR products: Blue/Green refers products 1 and 3; Blue/Red is 1 and 4; Orange/Green is 2 and 3; Orange/Red is 2 and 4. B. Average RCN values were calculated for each dog and the experiment repeated four times so that values could be compared to each other. All three means were shown to be different from each other (p << 0.05).