TABLE 2.
Kinetic parameters of the L. reuteri 121 GTFB-ΔN enzyme with the amylose V substratea
| Activity | Km (g · liter−1) | kcatb (s−1) | kcat/Km (g−1 · s−1 · liter) |
|---|---|---|---|
| Hydrolytic | 0.50 (±0.04) | 9.06 ± 0.18 | 18.5 (±1.8) |
| Transferase | 0.69 (±0.07) | 36.22 ± 1.14 | 53.0 (±7.2) |
| Total | 0.64 (±0.02) | 45.04 ± 1.14 | 70.6 (±4.0) |
a
A fixed amount of GTFB-ΔN enzyme (60 nM) was used with various concentrations of amylose V (0.025 to 0.4%, wt/vol) at pH 5.0 and 40°C. The values of the kinetic parameters presented are means ± standard deviations.
b
A molecular mass of 99 kDa (GTFB-ΔN) was used in the calculation of kcat.