TABLE 2.
Hybrid thermophilic n-butanol pathway enzymes
| Source thermophilic microorganism | Growth Topt (°C) | Enzyme | Query genea | Thermophile locus tag | Homology to query (Cov-ID-Pos)e | Monomer MMb (kDa) |
Purityc (%) | Enzyme MMd (kDa) | Molecular assembly | |
|---|---|---|---|---|---|---|---|---|---|---|
| Sequence-predicted | SDS-PAGE | |||||||||
| Caldanaerobacter subterraneus subsp. tengcongensis | 75 | Thl | CA_C2873 | TTE0549 | 99-68-83 | 43.6 | 42.3 | 98 | 158 | α4 |
| 75 | Hbd | CA_C2708 | TTE0548 | 99-70-84 | 32.8 | 34.4 | 86 | 206 | α6 | |
| 75 | Crt | CA_C2712 | TTE0544 | 98-60-80 | 30.0 | 29.7 | 79 | 92 | α3 | |
| Spirochaeta thermophila | 68 | Ter | TDE_0597 | STHERM_c16300 | 98-56-73 | 45.4 | 47.1 | 83 | 43 | α1 |
| Clostridium thermocellum | 60 | AdhE | CA_P0035 | Cthe_0423 | 99-64-82 | 97.2 | 94.3 | 81 | 254 | α3 |
| Thermoanaerobacter sp. strain X514 | 60 | AdhE | CA_P0035 | Teth514_0627 | 99-53-72 | 97.7 | 94.9 | 79 | 535 | α6 |
| 60 | Bad | Cbei_3832 | Teth514_1942 | 92-50-68 | 51.7 | 52.4 | 88 | 265 | α5 | |
| 60 | Bdh | CA_C3298 | Teth514_1935 | 99-29-49 | 44.0 | 40.4 | 82 | 79 | α2 | |
a
CA, Clostridium acetobutylicum; TDE, Treponema denticola; Cbei, Clostridum beijerinckii.
b
MM, molecular mass.
c
Determined by SDS-PAGE image densitometry.
d
Determined by gel filtration chromatography.
e
Cov-ID-Pos, percent coverage, percent identity, and percent positives.