TABLE 6.

Enzyme kinetic parameters used in model^a

Enzyme	Rate law	kcatfwd (s−1)	kcatrev (s−1)	Kmb		KI (μM)	Keqc	Reference(s)
				Substrate	Concn(μM)
Thl	Bi bi ping pong	53.9	987d	C2-CoA	271		2.8 × 10−5	This work; 74
				AcAc-CoA	33	133
				CoA	15	73
Hbd	Irreversible bi	35		AcAc-CoA	18.7		(180)	This work
Crt	Uni uni	267	267e	3HB-CoA	107		0.22	This work
				Crot-CoA	30			75
Ter	Irreversible bi	47.7		Crot-CoA	49.9		(1.2 × 109)	This work
				NADH	38.9
X514-Bad	Ordered bi ter	4.54 (C2-CoA)	116 (C2)f	C2-CoA	83.6		0.14 M	This work
				C4-CoA	27.2
		22.7 (C4-CoA)	573 (C4)f	C2	3,700		6.0 M	47
				C4	3,700
X514-Bdh	Irreversible bi	7.7 (C2)		C2	3,330		(790)	This work
		10.4 (C4)		C4	2,030		(2,200)
Ctherm-AdhE	Ordered bi ter	3.3 (C2-CoA)	82.2 (C2)f	C2-CoA	11.5		0.14 M	This work
		2.3 (C4-CoA)	57.8 (C4)f	C4-CoA	15.0		6.0 M	47
	Irreversible bi	3.6 (C2)		C2	7,760		(790)	This work
		15.4 (C4)		C4	6,920		(2,200)
X514-AdhE	Ordered bi ter	3.6 (C2-CoA)	90.3 (C2)f	C2-CoA	9.4		0.14 M	This work
		2.3 (C4-CoA)	56.8 (C4)f	C4-CoA	5.71		6.0 M	47
	Irreversible bi	23.0 (C2)		C2	22,000		(790)	This work
		22.9 (C4)		C4	3,230		(2,200)

^aAbbreviations: C₂-CoA, acetyl-CoA; AcAc-CoA, acetoacetyl-CoA; 3HB-CoA, 3-hydroxybutyryl-CoA; Crot-CoA, crotonyl-CoA; C₂-CoA, butyryl-CoA; C₂, acetaldehyde; C₄, butyraldehyde; CoA, coenzyme A.

^bK_m values for NADH, NAD, and CoA are assumed to be 50 μM unless otherwise specified.

^cEquilibrium constants calculated using eQuilibrator (76) at pH 7.9 and ionic strength of 0.1 M. The italic values shown in parentheses do not affect the reaction rate, because the reaction is modeled as irreversible.

^dBased on reference 74, assuming a constant ratio of k_cat^fwd to k_cat^rev.

^eAssumed to be equal to k_cat^fwd.

^fBased on reference 47, assuming a constant ratio of k_cat^fwd to k_cat^rev.