TABLE 1.
MLVA genotyping results for C. burnetii samples collected from vaginal mucus and feces in a French ovine flock between 2010 and 2011
| Ewea | Matrixb | Samplingf | Genotyping resultc |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Panel 1 (Ms01 to Ms36) |
Panel 2 (Ms23 to Ms34) |
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| Ms01 | Ms03 | Ms07 | Ms12 | Ms20 | Ms21 | Ms22 | Cox3, Ms26 | Ms30 | Ms36 | Ms23 | Cox4, Ms24 | Cox2, Ms27 | Cox5, Ms28 | Cox7, Ms31 | Cox6, Ms33 | Cox1, Ms34 | |||
| 1d | VM | 1 | 1 | 4 | 7 | 7 | 7 | 15 | 6 | NA | 12 | 6 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| F | 5 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 14 | 2 | 4 | 3 | 99 | 3 | |
| VM | 8 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | NA | 3 | 3 | 3 | 99 | 3 | |
| 2d | VM | 1 | 4 | 7 | 7 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| F | 1 | 4 | 7 | 8 | 7 | 15 | 6 | 8 | −1 | 12 | 4 | 99 | 14 | 2 | 4 | 4 | 99 | 3 | |
| VM | 4 | 4 | 7 | 7 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 | |
| VM | 7 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | 4 | 12 | 4 | 99 | 14 | 3 | 4 | 3 | 99 | 4 | |
| VM | 10 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 14 | 3 | 3 | 3 | 99 | 3 | |
| VM | 13 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 14 | 3 | 4 | 3 | 99 | 4 | |
| 3d | VM | 9 | NT | NT | NT | 4 | NT | NT | NT | NT | 12 | NA | 99 | 15 | 3 | 4 | 4 | 99 | 4 |
| 4d | VM | 5 | NA | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | NA | 3 | 5 | 3 | 99 | 4 |
| 5d | VM | 1 | 4 | 7 | 7 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| 6d | VM | 1 | 4 | 7 | 7 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| 7d | VM | 7 | NT | NT | NT | 7 | NT | NT | NT | NT | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| 8d | VM | 6 | NA | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | NA | 3 | 3 | 3 | 99 | 3 |
| 9 | VM | 4 | 9 | 4 | 7 | NA | 7 | 15 | 6 | 6 | 12 | 6 | 99 | 9 | NA | 4 | 3 | 99 | 3 |
| 10 | VM | 1 | 10 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | 12 | 6 | 99 | NA | 3 | 4 | 3 | 99 | 3 |
| 11 | VM | 1 | NT | NT | NT | 7 | NT | NT | NT | NT | 12 | 4 | 99 | 15 | 4 | 4 | 4 | 99 | 4 |
| 12 | VM | 6 | 4 | 7 | NA | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | NA | 3 | 4 | 3 | 99 | 3 |
| 13 | VM | 1 | 4 | 7 | 7 | 7 | 15 | 6 | NA | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| VM | 3 | NT | NT | NT | 7 | NT | NT | NT | NT | NA | NA | 99 | NA | 3 | 4 | 4 | 99 | 4 | |
| 14 | VM | 1 | NA | 7 | NA | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 15 | 3 | 4 | 3 | 99 | 4 |
| 15 | VM | 1 | NA | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 14 | 3 | 4 | 3 | 99 | 4 |
| 16 | VM | 1 | 4 | 7 | 8 | NA | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 7 | 3 | 4 | 3 | 99 | 3 |
| 17 | VM | 1 | NA | 7 | NA | NA | 15 | 6 | 6 | −1 | 10 | 4 | 99 | NA | 3 | 4 | 3 | 99 | 3 |
| 18 | VM | 1 | NA | 7 | NA | 7 | 15 | 4 | 6 | −1 | 10 | 4 | 99 | NA | 3 | 5 | 3 | 99 | 4 |
| 19 | VM | 1 | NA | 7 | NA | 7 | 15 | 6 | 6 | −1 | 12 | NA | 99 | NA | 3 | 4 | 3 | 99 | 3 |
| 20 | VM | 1 | 4 | 7 | 8 | 7 | 15 | 6 | 6 | −1 | 12 | 4 | 99 | 14 | 2 | 4 | 3 | 99 | 3 |
| Refe | 4 | 7 | 8 | 8 | 15 | 6 | 6 | 4 | 12 | 4 | 9 | 27 | 4 | 6 | 5 | 9 | 5 | ||
Ewes 4, 9, 10, and 20 are primiparous; all others are multiparous.
VM, vaginal mucus; F, feces.
Panels are as described by Arricau-Bouvery et al. (32). −1, deletion; 99, insertion of IS1111 gene; NT, not tested due to low DNA volumes (partial genotypes, only 10 markers tested); NA, not amplified. Ms nomenclature is as described by Arricau-Bouvery et al. (32); Cox nomenclature is as described by Svraka et al. (54).
Aborting female.
Nine Mile RSA 493 reference (Ref) strain.
Sampling period is shown as the week after abortion/parturition.