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. 2015 Sep 22;81(20):6953–6963. doi: 10.1128/AEM.01704-15

FIG 2.

FIG 2

Heterologous expression of E. coli ompF converts resistant strains into MccPDI-susceptible strains. (A) Western blot detection of E. coli OmpF synthesis in Salmonella enteritidis (strain 22577) and Yersinia enterocolitica (strain JB580v). Lane 1, wild-type strains; lane 2, Salmonella 22577(Ec.ompF) and Yersinia JB580v(Ec.ompF) without IPTG induction; lane 3, Salmonella 22577(Ec.ompF) and Yersinia JB580v(Ec.ompF) after induction with IPTG. (B) Competition with E. coli 25ΔtraM (microcin-producing) and E. coli 25ΔmcpM (no microcin production, negative control) after heterologous expression of E. coli ompF in Salmonella and Yersinia. Results are expressed as the difference between mean log CFU (± SEM; n = 3 independent experiments) during coculture and monoculture. *, significant ANOVA (P < 0.001) for comparisons between paired competitions (E. coli 25ΔtraM strain versus E. coli 25ΔmcpM strain).