TABLE 1.
Bacterial strains used in this study
| Strain name | Designation | Relevant genotype/phenotypea | Reference or source |
|---|---|---|---|
| E. coli 25 | E25 | Wild type; SSuTr PDI+ | 1 |
| E25ΔtraM | E25ΔtraM | SSuTr Cmr PDI+, traM deletion prevents conjugation | 3 |
| E25ΔmcpM | E25ΔmcpM | SSuTr Cmr PDI−, mcpM deletion eliminates PDI inhibition phenotype | 3 |
| Keio collection | |||
| BW25113 | Kwt | Nalr, Keio collection wild-type K-12 strain | 46 |
| BW25113 (pMMB207 only) | Kwt(vector ctrl) | Nalr Cmr, BW25113 with empty pMMB207 vector | This study |
| 51H12 ΔatpA (p207::atpA) | KΔatpA(atpA) | Kanr Cmr, atpA deletion complemented with atpA | This study |
| 53B1 ΔatpF (p207::atpF) | KΔatpF(atpF) | Kanr Cmr, atpF deletion complemented with atpF | This study |
| 65F11 ΔdsbA (p207::dsbA) | KΔdsbA(dsbA) | Kanr Cmr, dsbA deletion complemented with dsbA | This study |
| 67G8 ΔdsbB (p207::dsbB) | KΔdsbB(dsbB) | Kanr Cmr, dsbB deletion complemented with dsbB | This study |
| 7G4 ΔompF (p207::ompF) | KΔompF(ompF) | Kanr Cmr, ompF deletion complemented with ompF | This study |
| 5F1 ΔompR (p207::ompR) | KΔompR(ompR) | Kanr Cmr, ompR deletion complemented with ompR | This study |
| 7G4 ΔompF (p207::dsbA) | KΔompF(dsbA) | Kanr Cmr, ompF deletion expressing recombinant dsbA | This study |
| 7G4 ΔompF (p207 only) | KΔompF(vector ctrl) | Kanr Cmr, ompF deletion with empty pMMB207 vector | This study |
| 51H12 ΔatpA (p207::ompF) | KΔatpA(ompF) | Kanr Cmr, atpA deletion expressing recombinant ompF | This study |
| 53B1 ΔatpF (p207::ompF) | KΔatpF(ompF) | Kanr Cmr, atpF deletion expressing recombinant ompF | This study |
| 65F11 ΔdsbA (p207::ompF) | KΔdsbA(ompF) | Kanr Cmr, dsbA deletion expressing recombinant ompF | This study |
| 67G8 ΔdsbB (p207::ompF) | KΔdsbB(ompF) | Kanr Cmr, dsbB deletion expressing recombinant ompF | This study |
| 5F1 ΔompR (p207::ompF) | KΔompR(ompF) | Kanr Cmr, ompR deletion expressing recombinant ompF | This study |
| O157:H7 Sakai | O157:H7 | Wild type; Nalr | 47 |
| ΔatpA | O157:H7ΔatpA | Nalr Kanr, atpA deletion | This study |
| ΔatpF | O157:H7ΔatpF | Nalr Kanr, atpF deletion | This study |
| ΔdsbA | O157:H7ΔdsbA | Nalr Kanr, dsbA deletion | This study |
| ΔdsbB | O157:H7ΔdsbB | Nalr Kanr, dsbB deletion | This study |
| ΔompF | O157:H7ΔompF | Nalr Kanr, ompF deletion | This study |
| ΔompR | O157:H7ΔompR | Nalr Kanr, ompR deletion | This study |
| ompF heterologous expression, chimeric expression, and in cis His tagged | |||
| 7G4 ΔompF (p207::Se.ompF) | KΔompF(Se.ompF) | Kanr Cmr, ompF deletion complemented with Salmonella ompF | This study |
| 7G4 ΔompF (p207::ompFEc1–72+Se68–363) | KΔompF(ompFEc+Se) | Kanr Cmr, ompF deletion complemented with N-terminal sequence of E. coli ompF (bases 1–72) and C-terminal sequence (bases 68–363) of Salmonella ompF | This study |
| 7G4 ΔompF (p207::ompFSe1–68+Ec73–362) | KΔompF(ompFSe+Ec) | Kanr Cmr, ompF deletion complemented with N-terminal sequence of Salmonella ompF (bases 1–68) and C-terminal sequence (bases 73–362) of E. coli ompF | This study |
| 7G4 ΔompF (p207::Ec.ompFΔSYGG) | KΔompF(Ec.ΔSYGG) | Kanr Cmr, ompF deletion complemented with E. coli ompF sequence containing an SYGG deletion | This study |
| 7G4 ΔompF (p207::Ec.ompFΔKGN) | KΔompF(Ec.ΔKGN) | Kanr Cmr, ompF deletion complemented with E. coli ompF sequence containing a KGN deletion | This study |
| S. enteritidis 22577(p207::Ec.ompF) | Se22577(Ec.ompF) | Nalr Cmr, Se22577 expressing recombinant E. coli ompF | This study |
| Y. enterocolitica JB580v(p207::Ec.ompF) | JB580v(Ec.ompF) | Nalr Cmr, JB580v expressing recombinant E. coli ompF | This study |
| BW25113::ompF-His | Kwt::ompF-His | Nalr Kanr, in cis His-tag insertion at C-terminus of ompF | This study |
| S. enteritidis 22577::ompF-His | Se22577::ompF-His | Nalr Kanr, S. enteritidis strain 22577 with in cis His tag insertion at C terminus of ompF | This study |
| Other | |||
| BW25113ΔtonBΔtolA | KΔtonBΔtolA | Kanr Cmr, tonB and tolA deletion strain, BW25113 background | This study |
| BW25113ΔtonBΔtolQ | KΔtonBΔtolQ | Kanr Cmr, tonB and tolQ deletion strain, BW25113 background | This study |
| BW25113ΔexbDΔtolQ | KΔexbDΔtolQ | Kanr Cmr, exbD and tolQ deletion strain, BW25113 background | This study |
| BW25113ΔexbDΔtolR | KΔexbDΔtolR | Kanr Cmr, exbD and tolR deletion strain, BW25113 background | This study |
| S. enteritidis 22577 | Se22577 | Wild type, Nalr, Salmonella enterica serovar Enteritidis 22577 | This study |
| S. enteritidis 21679 | Se21679 | Wild type, Nalr, Salmonella enterica serovar Enteritidis 21679 | This study |
| Y. enterocolitica JB580v | JB580v | Nalr, Yersinia enterocolitica strain JB580v | 48 |
a
SSuTr, resistant to streptomycin, sulfonamide, and tetracycline antibiotics.