TABLE 3.

Gene knockout strains from the Keio collection that are no longer susceptible to killing by E. coli 25

Strain IDa	Gene knockout	Strain or gene functionb	Log10 CFU/ml ± SEM recovered after coculture withc:
			E. coli 25	E. coli 25ΔmcpM
BW25113	None	Wild-type E. coli K-12 from Keio collection; positive control	3.48 ± 0.30	8.20 ± 0.04
51H12	atpA	ATP synthase, F1 complex, α subunit; also called papA	5.61 ± 0.20	6.57 ± 0.26
53B1	atpF	ATP synthase, Fo complex, β subunit; also called papF	6.81 ± 0.39	7.33 ± 0.17
65F11	dsbA	Periplasmic protein disulfide isomerase; involved with disulfide bond formation	7.37 ± 0.06	8.22 ± 0.11
67G8	dsbB	Disulfide oxidoreductase; involved with disulfide bond formation membrane protein; oxidizes periplasmic DsbA	7.60 ± 0.38	8.33 ± 0.17
7G4	ompF	Outer membrane protein, porin	6.98 ± 0.14	7.97 ± 0.08
5F1	ompR	Transcriptional regulatory protein OmpR; response regulator for osmoregulation	6.33 ± 0.33	8.27 ± 0.07

^aStrain identifier (ID) or Keio collection clone ID.

^bPutative gene function as defined in the Ecogene 3.0 database using the structural and functional annotation of the E. coli K-12 genome (http://ecogene.org).

^cAverage CFU (±SEM) recovered after coculture with E. coli 25 (n = 3 independent replicates); recovery of >5 log₁₀ CFU/ml was considered resistant to MccPDI.