TABLE 3.

Properties of the genes present in the T. aromatica AR-1 3,5-DHB degradation cluster and their gene products

Gene	Properties					Related gene product
	Protein accession no.	% GC content	Distance to next gene (bp)	Product size (amino acids/kDa)	Product pI	% similaritya	% identityb	E value	Accession no.	Hypothetical function of closest homolog	Organism
orf1	AIO06081	69	8	410/44.8	9.07	68	74	2e−148	ACK54553.1	Glycosyltransferase	Thauera sp. MZ1T
orf2	AIO06100	65	626	260/29.8	7.38	74	83	5e−110	ACK54554.1	Metalophosphoesterase	Thauera sp. MZ1T
orf3	AIO06082	67	204c	308/33.2	9.59	53	56	1e−63	EED66667.1	LysR-type transcriptional regulator	Comamonas testosteroni
orf5	AIO06101	70	2	576/60.5	6.38	69	100	0.0	CAA12243.2	Oxoglutarate-ferredoxin oxidoreductase, α subunit	Thauera aromatica
orf6	AIO06083	70	376	307/32.6	6.70	79	97	4e−157	CAD27440.1	Oxoglutarate-ferredoxin oxidoreductase, β subunit	Thauera aromatica
dbhL	AIO06084	65		981/110.6	7.02	60	60	0.0	ABK58620.1	Resorcinol hydroxylase, α subunit	Azoarcus anaerobius
dbhS	AIO06085	65	15	288/32.3	7.10	58	53	3e−79	ABK58619.1	Resorcinol hydroxylase, β subunit	Azoarcus anaerobius
orf9	AIO06103	62	20	137/15.9	5.65	50	29	5e−06	EFW04169.1	Mannose-1-phosphate guanylyl transferase	Coprobacillus sp. 29_1
orf10	AIO06086	68	30	114/11.9	6.06	45	28	0.35	EEP26949.1	Unknown	Abiotrophia defectiva
btdL	AIO06087	74	102	293/29.5	6.0	63	50	8e−69	ABM15958.1	6-Phosphogluconate dehydrogenase	Mycobacterium vanbaalenii
orf12	AIO06088	67	98	417/46.5	5.50	68	93	0.0	ABK58632.1	M24 peptidase family	Azoarcus anaerobius
dbtP	AIO06102	68	72	321/35.2	6.99	53	40	2e−56	CAL95041.1	TRAP transporter, periplasmic protein	Azoarcus sp. BH72
dbtQ	AIO06104	67	4	163/17.8	9.73	52	34	8e−07	CAL95042.1	TRAP transporter, small permease	Azoarcus sp. BH72
dbtM	AIO06105	64	123	423/44.4	5.21	64	50	6e−95	CAL95043.1	TRAP transporter, permease	Azoarcus sp. BH72
orf16	AIO06089	66	100	344/37.9	7.77	66	57	2e−108	ACB33601.1	TRAP transporter, extracytoplasmic receptor	Leptothrix cholodnii sp-6
orf17	AIO06090	74	382	237/25.3	7.68	65	60	6e−72	EFI59106.1	αβ-Hydrolase	Comamonas testosteroni
orf18	AIO06091	67	64	716/80.0	6.16	60	53	0.0	CAJ71291.1	Molybdopterin oxidoreductase	“Candidatus Kuenenia stuttgartiensis”
bqdM	AIO06092	69	193	467/49.9	6.26	80	75	0.0	BAI72155.1	Dihydrolipoamide dehydrogenase	Azospirillum sp. B510
orf20	AIO06093	68	89	499/53.0	5.49	83	72	0.0	ACD96487.1	Succinic semialdehyde dehydrogenase	Geobacter lovleyi SZ
orf21	AIO06094	70	71	354/38.5	6.21	59	46	2e−81	ABK58627.1	p47k family protein	Azoarcus anaerobius
bqdL	AIO06095	70	12	735/78.7	5.64	75	74	0.0	ABK58621.1	Benzoquinone dehydrogenase, α subunit	Azoarcus anaerobius
orf23	AIO06096	68	32	182/20.2	5.95	72	73	1e−74	ABK58617.1	Glyoxalase	Azoarcus anaerobius
bqdS	AIO06106	72	52	437/44.5	5.98	65	54	5e−121	CAM75497.1	Pyruvate/α-ketoglutarate dehydrogenase complex, E2 component	Magnetospirillum gryphiswaldense
dbdR	AIO06107	68	134	317/34.5	7.20	52	33	1e−36	AAZ63058.1	LysR-type transcriptional regulator	Ralstonia eutropha
qorA	AIO06097	71	232	335/34.9	8.42	75	68	9e−148	EHP44701.1	Oxidoreductase	Cupriavidus basilensis
orf27	AIO06098	74	233	145/15.9	8.6	84	79	5e−81	ACK53423.1	Thioredoxin	Thauera sp. MZ1T
orf28d	AIO06099	72	92			78	83	0	ACK53440.1	(S)-Mandelate dehydrogenase	Thauera sp. MZ1T

^aAverage percent similarity to the 20 closest relatives in the databases, as determined with Blast2GO.

^bPercent identity with the closest homolog.

^cDistance to a pseudogene with no stop codon.

^dData for the available partial sequence.