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Effects of heat and digestive enzyme treatments on SEs. (A) SEs and BSA were maintained at 100°C. After the heat treatment, each sample was analyzed by SDS-PAGE. (B) Each protein (100 μg/ml) was incubated with pepsin (10 μg/ml) and analyzed by SDS-PAGE. (C) Each protein (100 μg/ml) was incubated with trypsin (100 μg/ml) and analyzed by SDS-PAGE. NT, no treatment; P, pepsin; Tr, trypsin.
