Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Nucleotide sequences and predicted sizes of PCR products
| Primer use and gene | Primer | Oligonucleotide sequence (5′-3′) | PCR product size (bp) |
|---|---|---|---|
| Real-time PCR | |||
| gyrB | gyrBF | AGGTCTTGGAGAAATGAATG | 113 |
| gyrBR | CAAATGTTTGGTCCGCTT | ||
| sely | SElYF | CAATGTACGGACAGTGCTCTACAA | 189 |
| SElYR | TGACCGTTAACAAACAAGTTCATTC | ||
| Cloning, sely | SElYGSTF | CCCCGGATCCAAAACAACTGGATTGATTA | 599 |
| SElYGSTR | CCCCGTCGACCTATTTCATATAAATATCT |
