Figure 3.

Definition and specificities of the identified TSSs. (a) Details of annotation and classification of 5029 TSS into 1472 putative gTSS giving rise to mRNA, 1460 aTSS producing asRNA, 1478 iTSS for internal sense transcripts and 619 nTSS for candidate ncRNAs. aTSS was classified as gTSS when the TSS was located 1–200 nt upstream and in the same orientation as an open reading frame (ORF). TSSs located antisense to an annotated gene or within ≤50 bp 5′ or 3′ to it on the reverse strand were classified as aTSS. TSSs positioned within an annotated gene were classified as iTSS and all remaining TSSs as nTSS from which ncRNAs originate. (b) Overlaps and specificities of the different types of TSSs in the three compared conditions.