Table 2. Network analysis on stepwise-added metagenomes.
# Metagenomes | 1a | 2b | 3c | 4d | 5e | 6f | 7g | 8h | 9i |
---|---|---|---|---|---|---|---|---|---|
Contigs | 211 | 2202 | 6148 | 9963 | 14 741 | 19 977 | 24 527 | 28 134 | 30 132 |
Edges | 942 | 10 012 | 36 665 | 68 197 | 121 704 | 186 584 | 237 728 | 277 340 | 304 585 |
Average degree | 8.929 | 9.094 | 11.927 | 13.690 | 16.512 | 18.680 | 19.385 | 19.716 | 20.217 |
Modularity | 0.682 | 0.923 | 0.946 | 0.957 | 0.956 | 0.956 | 0.956 | 0.959 | 0.959 |
Viral groups | 8 | 28 | 55 | 68 | 81 | 84 | 92 | 103 | 105 |
Avg. clustering coefficient | 0.688 | 0.720 | 0.750 | 0.765 | 0.770 | 0.768 | 0.766 | 0.766 | 0.766 |
# Metagenomes | 1j | 2k | 3l | 4m | 5n | 6o | 7p | 8q | 9r |
Contigs | 509 | 3583 | 9120 | 13 995 | 17 776 | 22 889 | 26 618 | 30 132 | |
Edges | 1344 | 12 711 | 39 773 | 78 338 | 115 037 | 178 631 | 238 101 | 304 585 | |
Average degree | 5.281 | 7.095 | 8.722 | 11.195 | 12.943 | 15608 | 17.89 | 20.217 | |
Modularity | 0.900 | 0.962 | 0.969 | 0.962 | 0.965 | 0.962 | 0.961 | 0.958 | |
Viral groups | 19 | 51 | 86 | 94 | 106 | 108 | 105 | 105 | |
Avg. clustering coefficient | 0.650 | 0.712 | 0.730 | 0.742 | 0.751 | 0.758 | 0.764 | 0.766 |
0809.
0809-0901.
0809-0903.
0809-0904.
0809-0908.
0809-0909.
0809-0910.
0809-1002.
0809-1006.
Did not have a major population.
1006-1002.
1006-0910.
1006-0909.
1006-0908.
1006-0904.
1006-0903.
1006-0901.
1006-0809.
The 9 454 metagenomes were added one-by-one successively to the previous metagenome (1, 1+2, 1+2+3, etc.) and analyzed using the network-based analysis. The number of contigs present in the network is given under ‘Contigs' with the number of connections between all contigs as ‘Edges.' Average degree refers to the average number of connections incoming/outgoing from each contig and denotes relative connectivity between contigs. Modularity is a measure of separation and distinction of viral groups. The greater the value (approaches 1) the more connections tend to exist between members within a viral group than between members of differing viral groups. This also serves as a measure of the overall organizational level of the network, with values >0.3 indicating community structure. The average clustering coefficient measures how well contigs tend to associate with each other.