Table 4. Summary OTU statistics.
|
Pre-coloniser |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | V. comedens | H. fragiforme | Biscogniauxia sp. | T. versicolor | S. hirsutum | B. adusta | H. fasciculare | ||
| Replicates included in analysis | 8 | 9 | 9 | 8 | 8 | 8 | 9 | 4 | |
| No. OTUs pre-rarefaction (including pre-coloniser) | Total | 373 | 299 | 332 | 272 | 235 | 170 | 200 | 150 |
| Mean counts per sample | 6149 | 4194 | 5339 | 4421 | 3785 | 1545 | 3433 | 2558 | |
| Mean OTUs per sample | 79 | 61 | 65 | 53 | 50 | 40 | 45 | 48 | |
| No. OTUs after rarefaction (inlcuding pre-coloniser) | Total | 88 | 71 | 79 | 64 | 58 | 58 | 58 | 42 |
| Mean counts per sample | 205.5 | 205.9 | 205.4 | 206.3 | 206.4 | 206.6 | 206.7 | 206.3 | |
| Mean OTUs per sample | 19.3 | 16.6 | 16.7 | 11.0 | 15.0 | 14.1 | 15.0 | 14.5 | |
| No. OTUs pre-rarefaction (excluding pre-coloniser) | Total | 373 | 298 | 329 | 273 | 231 | 165 | 196 | 147 |
| Mean counts per sample | 6149 | 4058 | 5234 | 4421 | 3008 | 783 | 2479 | 3869 | |
| Mean OTUs per sample | 79 | 60 | 64 | 53 | 49 | 39 | 43 | 67 | |
| No. OTUs after rarefaction (excluding pre-coloniser) | Total | 88 | 70 | 78 | 64 | 57 | 57 | 57 | 41 |
| Mean counts per sample | 205.5 | 175.9 | 192.4 | 206.3 | 111.1 | 89.0 | 142.9 | 113.5 | |
| Mean OTUs per sample | 19.3 | 15.9 | 16.1 | 11.0 | 14.1 | 13.1 | 14.1 | 14.0 | |
| Pre-coloniser present? | Y/N | — | Y | Y | N | Y | Y | Y | Y |
| % total OTU countsa | — | 14.6 | 6.3 | 0 | 46.15 | 56.93 | 30.68 | 45 | |
| Ascomycota | No. | 62 | 53 | 57 | 43 | 39 | 37 | 35 | 27 |
| % total OTUs | 70.5 | 75.7 | 73.1 | 67.2 | 68.4 | 64.9 | 61.4 | 65.9 | |
| % total counts | 62.9 | 18.5 | 65.2 | 56.8 | 77.3 | 72.9 | 72.3 | 34.1 | |
| Basidiomycota | No. | 21 | 12 | 19 | 19 | 12 | 16 | 18 | 10 |
| % total OTUs | 23.9 | 17.1 | 24.4 | 29.7 | 21.1 | 28.1 | 31.6 | 24.4 | |
| % total counts | 34.9 | 59.3 | 34.5 | 43.1 | 19.2 | 18.8 | 25.7 | 63.9 | |
| Unidentified | No. | 5 | 5 | 2 | 2 | 6 | 4 | 4 | 4 |
| % total OTUs | 5.7 | 7.1 | 2.6 | 3.1 | 10.5 | 7.0 | 7.0 | 9.8 | |
| % total counts | 2.3 | 4.7 | 0.3 | 0.1 | 3.5 | 8.3 | 2.0 | 2.0 | |
| Dominant replacing OTU | Name | Lasiosphaeris hispida | Helotiales sp. | Lasiosphaeris hispida | Lasiosphaeris hispida | Phialocephala dimorphospora | Phialocephala dimorphospora | Xenasmatella vaga | Xenasmatella vaga |
| % total OTU counts | 26.6 | 18.5 | 18.8 | 21.0 | 26.1 | 27.0 | 19.7 | 52.4 | |
| Asco/basidiomycota | Ascomycota | Ascomycota | Ascomycota | Ascomycota | Ascomycota | Ascomycota | Basidiomycota | Basidiomycota | |
| Shannon diversity (H) | (F7,55=1.3, P=0.28) | 1.668 | 1.395 | 1.518 | 0.925 | 1.843 | 1.637 | 1.625 | 1.539 |
| Fisher's alpha (α) | (F7,55=0.9, P=0.51) | 5.379 | 4.456 | 4.394 | 2.673 | 5.722 | 5.085 | 4.532 | 4.675 |
| Pielou's evenness (E) | (F7,55=2.1, P=0.06) | 0.558 | 0.517 | 0.541 | 0.352 | 0.693 | 0.663 | 0.624 | 0.660 |
Abbreviations: ANOVA, analysis of variance; N, no; OTU, operational taxonomic unit; Y, yes.
Number of OTUs pre- and post rarefaction are given for data including and excluding OTUs corresponding to the pre-coloniser species; where there was no pre-coloniser (control) or the pre-coloniser was absent in the OTU profile (Biscogniauxia sp.), data are the same in both instances.
All other analyses were performed on data with pre-coloniser OTUs excluded. Results from one-way ANOVA comparisons of Shannon diversity (H), Fishers' alpha (α) and Pielou's evenness (E) are given as degrees of freedom (df), the F-statistic and P-value; no significant differences (P>0.05) were found in any of these measures between different pre-colonisers.
This corresponds to total OTU counts including the pre-coloniser species.