Table 5. Pairwise differences in community profiles following different pre-coloniser treatments using metagenomics (OTUs) vs traditional culture (cOTU) techniques.

Pairwise comparison	OTU profiles			cOTU profiles
	df	F	P (adj)	df	F	P (adj)
Control–V. comedens	16	2.510	0.014a	30	2.660	0.009a
Control–H. fragiforme	16	0.840	0.654	24	1.050	0.500
Control–B. nummularia	15	0.749	0.750	24	1.050	0.500
Control–T. versicolor	15	2.786	0.011a	31	3.070	0.009a
Control–S. hirsutum	15	3.545	0.011a	23	1.690	0.068
Control–B. adusta	16	2.839	0.012a	23	1.370	0.210
Control–H. fasciculare	11	2.193	0.026a	23	2.840	0.009a
Control–P. velutina	—	—	—	22	2.170	0.017a
Control–P. impudicus	—	—	—	22	0.910	0.721
V. comedens–H. fragiforme	17	1.844	0.031a	23	2.270	0.031a
V. comedens–B. nummularia	16	2.058	0.018a	23	2.060	0.035a
V. comedens–T. versicolor	16	1.818	0.022a	30	2.240	0.009a
V. comedens–S. hirsutum	16	2.377	0.011a	22	0.970	0.633
V. comedens–B. adusta	17	1.874	0.031a	22	1.830	0.061
V. comedens–H. fasciculare	12	1.412	0.144	22	2.290	0.02a
V. comedens–P. velutina	—	—	—	21	1.710	0.061
V. comedens–P. impudicus	—	—	—	21	2.050	0.042a
H. fragiforme–B. nummularia	16	0.661	0.873	17	0.740	0.930
H. fragiforme–T. versicolor	16	1.961	0.019a	24	2.160	0.009a
H. fragiforme–S. hirsutum	16	2.600	0.011a	16	1.420	0.103
H. fragiforme–B. adusta	17	2.029	0.052	16	1.180	0.340
H. fragiforme–H. fasciculare	12	1.553	0.120	16	2.040	0.015a
H. fragiforme–P. velutina	—	—	—	15	1.720	0.025a
H. fragiforme–P. impudicus	—	—	—	15	0.830	0.873
B. nummularia–T. versicolor	15	2.148	0.011a	24	1.750	0.03a
B. nummularia–S. hirsutum	15	2.675	0.012a	16	0.890	0.796
B. nummularia–B. adusta	16	2.432	0.016a	16	0.770	0.931
B. nummularia–H. fasciculare	11	1.875	0.017a	16	1.530	0.048a
B. nummularia–P. velutina	—	—	—	15	1.310	0.070
B. nummularia–P. impudicus	—	—	—	15	0.540	0.992
T. versicolor–S. hirsutum	15	1.439	0.017a	23	1.240	0.217
T. versicolor–B. adusta	16	1.439	0.144	23	1.570	0.066
T. versicolor–H. fasciculare	11	1.137	0.356	23	1.580	0.050
T. versicolor–P. velutina	—	—	—	22	0.780	0.931
T. versicolor–P. impudicus	—	—	—	22	1.820	0.0225a
S. hirsutum–B. adusta	16	2.175	0.019a	15	0.760	0.931
S. hirsutum–H. fasciculare	11	1.933	0.026a	15	1.290	0.204
S. hirsutum–P. velutina	—	—	—	14	0.800	0.931
S. hirsutum–P. impudicus	—	—	—	14	0.930	0.723
B. adusta–H. fasciculare	12	1.236	0.306	15	1.560	0.045a
B. adusta–P. velutina	—	—	—	14	0.980	0.721
B. adusta–P. impudicus	—	—	—	14	0.590	0.963
H. fasciculare–P. velutina	—	—	—	14	1.220	0.273
H. fasciculare–P. impudicus	—	—	—	14	1.840	0.0168a
P. velutina–P. impudicus	—	—	—	13	1.360	0.096

Abbreviations: df, degrees of freedom; PERMANOVA, permutational multivariate analysis of variance.

Comparisons were performed using Adonis/PERMANOVA, following which a P-adjustment was performed to reduce the effect of false discovery rates (Benjamini and Hochberg, 1995); adjusted P-values are shown.

^aSignificant difference in community composition (P<0.05).