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. Author manuscript; available in PMC: 2015 Sep 23.
Published in final edited form as: Methods Mol Biol. 2015;1250:349–361. doi: 10.1007/978-1-4939-2074-7_27

Table 2. Troubleshooting of the caspase 3-like activity measurement procedure.

Problem Cause Solution
Negative testing results Decomposed reaction buffer and solutions Prepare new reaction buffer and solutions and protect from light
Air bubbles in the wells of the black 96-well plate Tap the black 96-well plate before incubation in the multiplate reader
Divergent testing results Incomplete lysis of the cells Prepare new lysis buffer and strongly pipette up and down when lyzing cells
Variation in temperature Check temperature in the multiplate reader and ensure a constant temperature of 37°C
Variation in pH and crystallization of the reaction buffer Check the pH of the reaction buffer and adjust to 7.4