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. Author manuscript; available in PMC: 2015 Sep 23.
Published in final edited form as: Methods Mol Biol. 2015;1250:349–361. doi: 10.1007/978-1-4939-2074-7_27

Table 3. Troubleshooting of the lactate dehydrogenase (LDH) assay.

Problem Cause Solution
Negative testing results Decomposed LDH reaction buffer Prepare new LDH reaction buffer and store for a maximum of 5 days or 8 h at 4°C or 25°C, respectively
Air bubbles in the microcuvette Shake the microcuvette upon mixing the LDH reaction buffer and the sample
Divergent testing results Incomplete homogenization of samples Subject samples to an additional series of 20 ultrasonication pulses
Variation in temperature Check temperature and store the LDH reaction buffer at 25°C