Table 1.

Summary of data collection and structure refinement. Values for high resolution shells are shown in brackets. B36 = BRCC36, KIAA = KIAA0157, QSQ = BRCC36 H94Q + H96Q mutant.

	CfB36-KIAΔC	CfB36-KIAΔC	CfB36(QSQ)-KIAΔC	DrBRCC36
Substitution	None	SeMet	None	None
Space Group	P212121	P212121	P212121	P 6222
Wavelength (Å)	1.28240	0.97920	1.28270	0.97920
Unit cell (Å)
a	49.9	50.1	89.4	108.8
b	116.6	116.3	124.2	108.8
c	226.1	231.5	132.5	137.4
α = β	90°	90°	90°	90°
γ	90°	90°	90°	120°
Molecules/asu
BRCC36	2	2	2	1
KIAA0157	2	2	2	–
Resolution (Å)	2.55 (2.65-2.55)	2.54 (2.7-2.54)	2.75 (2.85-2.75)	3.2 (3.3-3.2)
Observed reflections	277108 (30610)	243872 (39265)	103925 (9417)	85408 (7589)
Unique reflections	80922 (8974)	84223 (13506)	37786 (3622)	8474 (816)
Redundancy	3.4 (3.4)	2.9 (2.9)	2.8 (2.6)	10.1 (9.3)
I/σI	7.2 (1.0)	14.3 (1.1)	7.3 (0.8)	12.7 (1.5)
Completeness (%)	97.3 (98.0)	98.1 (97.3)	95.8 (93.2)	99.9 (99.9)
Rmeas	0.123 (149.4)	0.059 (123.5)	0.158	0.201
CC1/2 (%)	(43.4)	(52.4)	(50.0)	(87.4)
Rwork, Rfree	0.211, 0.259	0.189, 0.240	0.231, 0.277	0.214, 0.241
Average B-factor (Å2)	76.5	93.7	72.9	82.3
RMSD from ideal geometry
bonds (Å)	0.002	0.003	0.002	0.002
angles (°)	0.505	0.554	0.493	0.527
Ramachandran plot statistics
Residues in favored region	97.0%	96.1%	98.2%	954.14%
Residues in allowed region	2.8%	3.8%	1.7%	4.9%
Residues in outlier region	0.2%	0.1%	0.1%	0.0%
PDB ID	–5CW3	5CW4–	5CW5–	5CW6–