Table 3. Neutralization of various HCMV clinical isolates by 1G2.
| HCMV virus strain |
Neutralization EC50s for 1G2 (μg ml−1) |
Residue at position 287 | |
|---|---|---|---|
| ARPE | NHDF | ||
| VR1814 (1G2 mAb) | 0.213 | 0.077 | Thr |
| VR1814 (1G2 Fab) | 0.151 | NA | Thr |
| TM-31354 | 0.230 | 0.411 | Thr |
| 8816 | 0.046 | NA | Ala |
| 8818 | 0.688 | 0.289 | Thr |
| 8819 | 0.485 | 0.850 | Ala |
| 8821 | 0.108 | 0.401 | Thr |
| 8824 | 0.019 | 0.043 | Ala |
| 8822 | 0.319 | 0.485 | Ala |
| TR | 0.248 | 0.303 | Ala |
| TM-28175 | NA | 0.138 | Ala |
| MP-MD-805 | NA | 0.115 | Thr |
| MP-LW-1802 | 0.104 | NA | Thr |
HCMV, human cytomegalovirus; NA, not applicable; NHDF, normal human dermal fibroblasts.
EC50 values for various clinical HCMV isolates measured on epithelial cells (ARPE-19) and fibroblasts (NHDF). Infection of 50% cells was determined based on HCMV IE expression. NA, clinical isolate did not express IE on a particular cell type.