Table 1.

Pairs of primers used for HRM screening, sequencing and genotyping ABCC1

	Exon	Number of scanned bp upstream from the exon (without primer)	Forward primer (5’ → 3’)	Reverse primer (5’ → 3’)	Number of scanned bp downstream from the exon (without primer)	Size (bp) including primers
Scanning	1	104*	CAGCGCTAGCGCCAGCAG	CCCGGTGCTTTCCCTCCC	49	234
	2	31	GTCCTCTGGGGTGTGTCCT	TCTGAATGTAGCCTCGGTCA		201
			GCTTTCAGAACACGGTCCTC	TTATCACCAACCACCACTCC	52	203
	3	20	GGGCGGTCTGTTGTAGGATA	CCACTGAGCCCAGCAGAT	31	215
	4	129	AAGCTGAGGCAGGAGAATCA	AAGGTAGCAAGCAGCTGAGG		163
			AGCCTGGGTGACAAGAGTGA	TGGATCTCAGGATGGCTAGG		189
			ATGCTCACTTTCTGGCTGGT	GGCAACGCATGACTTCTACA	72	170
	5	27	CAGCCCCAGAATGTGATCTT	CACACACACGCACACACACT	19	212
	6	20	TTTCCCTCTTCCTCCCAAAC	AGCTGAGCATGTTCATTCGTT	59	182
	7	33	CCCTCCTCCTGTCATTGACTC	GAGTGGAAAGGAGGTGACCA		240
			GGAACAAGTCGTGCCTGTTT	ATCTTGCCCAGAACCACAAA	104	184
	8	74	TTCCCTGAAGGGTGACATTC	AGGGGTTCCACTCCTTCTGT		219
			GGAGGCTTTGATCGTCAAGT	AAAGCCAAGGAGGGAAAATG	29	199
	9	14	AGTCATTCCAGGCCCTCTCT	TGACGAAGCAGATGTGGAAG		165
			TACACCGTGCTGCTGTTTGT	CAGGAGGGGATGTGGAAGT	33	175
	10	27	TCTTCAGCTGCCACACTCAC	GCCACAGGAGGTAGAGAGCA		207
			TCTGTGGACGCTCAGAGGTT	CTGCCCACACGTAGAAGTCC	38	157
	11	21	TCTTCTGTCTGGTGAGTGATGAA	TGGACTAAAATCCTCATGGAGAG	49	209
	12	41	GTTGAGTGATGGGCTGATCC	GGCAGACTTCTTCAGCACCT		217
			GCATTCAAGGACAAGGTGCT	CCTGGGCAACATAGTGACCT	75	206
	13	62	GTCTCCAGGGCCTGTCACT	ACCGGAGGATGTTGAACAAG		184
			CCAGACAGCCTTCGTGTCTT	GCTTTCGTGGCCTAGAACC	42	149
	14	49	TTAACCTTGGTTGGTTTTGC	CAAGTAGGGAGGACCCAGTG	51	228
	15	41	GTTCTGTGCATGTGGAGTCG	GGCTCTCTCCACACCATCAC	22	179
	16	73	TTCTCTACTTGGGGTAAATTGAGG	CTGAGAGCAGGGACGACTTT		177
			ATCCCCGAAGGTGCTTTG	GCTTTTCCTCAGACCACCAG	37	171
	17	12	CTGTCTCACCTCGTTCTCCA	CTGACCTTCTCGCCAATCTC		214
			CTCCCAGACCTGGAAATCCT	CAGCCCACTGAGATTGTGAG	60	134
	18	20	ATTTCCCAGGAAACCCACTC	GGCCAATCACATTTTCAAAGA		185
			CTTCGATGATCCCCTCTCAG	CCTAAAGGGGACACGTTCTG	55	166
	19	26	CTCACACATGTGCACTCACG	TCTGTGCTGGCATAGGTACG		204
			GCCAAGCTAGGCAGTCTCAC	GGCAAGTAGCTCATGCTGTG		99
			TCTGAGATGGGCTCCTACCA	CAACCTCAAAGAGGCCAATG	56	182
	20	48	TTGTTGCCCTTGGTTTTAGC	CTCCTCTTGTCCTCCCACTG	44	223
	21	20	GCATCTGTACGGTTGACACC	TGGGCGCTCTATAAACTCCA	32	228
	22	25	GGTGCGTGCATGTGCTAAG	ATGGGGTCATCAGTCCAGAG		184
			GGCTTCCAACTATTGGCTCA	CAAAACGCTGAGGACTCTAAGG	72	204
	23	21	CCCTCTCTGCATTGTGGAGT	GTCCACTGTGTCCAGCTCCT		214
			ATGAGCTTCTTTGAGCGGAC	GGCTTGTCCCGAACACTAAG	14	244
	24	22	GAGGGAACCTTCATCAACTCC	TCTGGTTCTCGTCCACCTTC		212
			CCCATTTCAACGAGACCTTG	GGAAAAGATCGAGGCAAAGA	55	208
	25	26	AGAGCTGACTCCATGCCTGT	GGCTCCCCCATTAACAGACT	32	225
	26	24	CGCCCGCTTACTCTAGAAAT	CCAAAGACCAAGAGGTCCAG	18	184
	27	45	GGGGAGTCACAGCTTTACCA	GGGAATGGGTGAGGGAAT	18	248
	28	6	ACACCTGGGCCCTTCTGTC	TGATCTCTCCTTCGGCAGAC		113
			CTGGGCTTATTTCGGATCAA	CAGTGCAATCATAGGGCTTG	40	174
	29	55	AGTCCTTAGGTCGCCTCCAT	CCTTCTGCACATTCATGGTC		227
			CCCACCTGAAGGACTTCGT	CAAACACCCCTACCGAGATG	47	143
	30	74	GTGTCTCCTTTCGCTTCTCC	ATGGTGGACTGGATGAGGTC		217
			CCTGCTGAGGAAGACGAAGA	AGGCAAACTCCCAAAGCCTA	25	204
	31	15	CCCTTCCCCTCATGTCTGTA	ATAGGCCCTGCAGTTCTGAC	21**	185
Sequencing	1		-	-		-
	2		TCTCGAACTCCCAGCCTAAA	TAAGCGGCAGAGCAAAGATT		749
	3		GAGCATGGTGACCAGACAAA	CTCCTGACCTCAGGTGATCC		638
	4		GTGGTGAAACCCCGTCTTTA	CCTTGGAGCAACACAGACAA		604
	5		CCCGAGTAGCTGGGATTACA	ACCATGCCAAGTGAGAAACC		606
	6		GGGTTGTTGTGGGGATAGAA	AAGCAAAAGCAGTCCAGCAT		622
	7		CTGCATGACCCAACAGAATG	CAAACTCCCGACCAAGTGAT		651
	8		CTTTGACTCTGCCTTCCCTG	TAGGGGAGCCTAGTGGGTTT		691
	9		AGCAATTAGTGCACAACCCC	AAGACATCTACACGCCCACC		613
	10		ACAGCCTGGAAGCGTAGAAA	AACTGCCAGGGAGCTCTACA		664
	11		GAGCGTGGACCTGCTTATTC	CACCGGCCTACAAGGTCTAA		608
	12		GATGTTGAGTGATGGGCTGA	CCTGGGCAACATAGTGACCT		363
	13		ATACTGCCCCAGGTTTTTCC	AAAAAGGATAGGAATGGCGG		611
	14		CTGGGGAAACCCTTGAAAGT	CCAAGGGAAAGAAATGCAAG		263
	15		CCCAAGATGATCGTCCAGAT	TACGCAAGCCAAGAATCTCC		632
	16		TCTCCTTTCAGACCTTGCGT	CCTGCCTTCTAGGACAGCAC		676
	17		ACTCTTTGGAAGCAGGGGTT	AGTGAGACCTGAGCCACACC		693
	18		CTCAAGCCATCTTCCCACTC	GAAGCCAGCCCTGTGACTTA		717
	19		CATGTCCCACCTTCAGACCT	CCAGCTTAACTCCGTGCTTC		748
	20		CTGCACAGTTGCAAAGCACT	GCCAAGAGACCTGAGCAAAG		620
	21		CCGTCTCTTATGCCATGGTT	GGTTCAAATCCCAGTTCTGC		461
	22		GCATGTGCTAAGCTGCCTTAT	TGTAAAATGGGCACACTGGA		485
	23		CTCAAGTGATCCACCCACCT	TGCTTCAAAAGCACCACACT		617
	24		GAGGTTAGCACTTTGCAGCC	ATGATTCATCTGTCCTCCGC		791
	25		AAGGCAAGCTTCAGATTCCA	AAAGCATTCCCCTCGTACCT		674
	26		AAATGCCACGTGACTCTTCC	CCTGGTGAGGTATCCAGCTC		242
	27		AGGGGACAGAGGGACACAG	AAATCTGTGGGGCTCATTTG		618
	28		TCATGATGGGAAACTCACCA	GAACGATGAAGTAGGGCCAA		669
	29		AGACAGGGTGTTGCCATGTT	TCAACTGAATGGAGCTGGTG		689
	30		GGTGTGAGCTGCTGTTTTGA	AAGAGAGGATCCACCCACTG		556
	31		CCATGATTGATGTGGGGTAG	AAGCACCAGGAAACCACTTG		640
Genotyping	SNP		Forward primer (5’ → 3’)	Reverse primer (5’ → 3’)		Size (bp) including primers
	c.128G > C		TCAGAACACGGTCCTCG	ACAGGCCCAGAGGTAAAA		49
	c.616-7942A > G		AGCAACAGGGCAAACAAATC	GACGGAGCCTAAATGTCCAG		76
	c.816G > A		CTTGTGTTCCAGGCAGC	GCAGGATCCTTGGAGGAGTA		51
	c.825 T > C
	c.1057G > A		GCTCCTTTGCAGGTTGCTCATC	GGGGCCTTCGTGTCATTCA		48
	c.1062 T > C		GGTTGCTCATCAAGTTCGTGA	GTCTGGGGCCTTCGTGTC		41
	c.1218 + 8A > G		CATGAGGATCAAGACCGCTG	GTGGAAGTCGGGCCACAT		84
	c.1299G > T		CCAGAAAATCCTCCACGGT	GTACGTGGCCAAGTCCATGA		80
	c.1684 T > C		GTTCTGCTTGCAGGAGGCC	TTCAGGGGAAACCGGAGGAT		125
	c.1704C > T
	c.2012G > T		TCACCTTCTCCATCCCCG	CACTTTGTCCATCTCAGCCA		104
	c.2168G > A		CAGGCCTGGATTCAGAATGA	ATGGTTCCTCCAGCTGACAT		67
	c.2581G > A		CAGGAGCTGCTGGCTCG	TCTGTGCTGGCATAGGTACG		62
	c.2965G > A		TCCTTTTCATGTGTAACCATGTG	CAATAGTTGGAAGCCAGCG		46
	c.3140G > C		CTACTCCATGGCCGTGTCC	AGGATGCTGTGCAGCAGGT		75
	c.3436G > A		GAGGTTCTACGTGGCTTCC	GAATAGACCGGGGAGCG		69
	c.4002G > A		GACAAGTCCGGATGCCAG	CGAAATAAGCCCAGGGTC		112
	c.4009A > G		AGCTGGGAAGTCGTCCCT	TGATCTCTCCTTCGGCAG		65

104* - upstream from the START codon. 21** - downstream from the STOP codon. Bold variants means these ones which genetic variation was detected during genotyping method