Table 1.
Transcript accumulation and activity of cell wall invertase, and sugar content in stem bases of cuttings of wild type, pHell and PhAOC-RNAi plants
| parameter | unit | WT | pHell | PhAOC-RNAi | |||
|---|---|---|---|---|---|---|---|
| line 4 | line 10 | line 4 | line 10 | line 15 | |||
| 3CL9414-transcripta | 2−∆Ct | 20.9 ± 10.1 | 19.4 ± 7.5 | 23.1 ± 3.0 | 19.2 ± 4.6 | 14.6 ± 7.2 | 17.8 ± 5.4 |
| invertase activityb | pkat/mg protein | 12.6 ± 3.7 | 14.1 ± 3.3 | 10.2 ± 5.2 | 10.1 ± 4.2 | 9.7 ± 3.9 | 8.3 ± 4.4 |
| glucosec | μmol/g f.w. | 0.2 ± 0.1 | 0.2 ± 0.0 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 |
| fructosec | μmol/g f.w. | 0.4 ± 0.3 | 0.2 ± 0.1 | 0.1 ± 0.0 | 0.5 ± 0.4 | 0.4 ± 0.4 | 0.5 ± 0.3 |
| sucrosec | μmol/g f.w. | 1.8 ± 0.6 | 1.6 ± 0.2 | 1.1 ± 0.6 | 2.7 ± 0.9 | 2.3 ± 0.7 | 2.4 ± 1.2 |
All data are given as mean values ± SD from eight independent, biological replicates. Data were compared by one-way-ANOVA and did not show significantly different values according to Tukey’s HSD test
aTranscript data are relative to PhRSP13 determined at 4 hpe
bCell wall invertase activity determined at 6 hpe
cSugar levels determined at 192 hpe