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. 2015 Jul 13;12(5):580–591. doi: 10.1038/cmi.2015.57

Table 2. The sequences of the primers used in the RT-PCR analysis.

Gene Forward primer (5′-3′) Reverse primer (5′-3′)
Foxp3 CAG CAC ATT CCC AGA GTT CCT C GCG TGT GAA CCA GTG GTA GAT C
CD25 GTC CTG GGA CAA CCA ATG TC TTC TGT TCT TCA GGT TGA GGT G
GITR GTT TTG GCT TCC AGT GTA TCG A AAC ACA GTG AGA AAC CCG AAC T
CTLA-4 CCG AAC TAA CTG CTG CAA GGA CCC AGA TTT ATG TAA TTG ATC CAG AA
CD28 ATG AAT CAG TGA CAT TCT ACC TCC AGG TGT TTC CCT TTC ACA TGG
IL-2 GAA TGG AAT TAA TAA TTA CAA GAA TCC C TGT TTC AGA TCC CTT TAG TTC CAG
TNF-α CCC CAG GGA CCT CTC TCT AAT C GGT TTG CTA CAA CAT GGG CTA CA
IFN-γ GTT TTG GGT TCT CTT GGC TGT TA AAA AGA GTT CCA TTA TCC GCT ACA TC
IL-10 GCT GGA GGA CTT TAA GGG TTA CCT CTT GAT GTC TGG GTC TTG GTT CT
TGF-β1 CAG CAA CAA TTC CTG GCG ATA C TCA ACC ACT GCC GCA CAA CT
β-actin GAG CTA CGA GCT GCC TGA CG GTA GTT TCG TGG ATG CCA CAG