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. 2015 Sep 23;46(1):101. doi: 10.1186/s13567-015-0231-y

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© Baron and Baron. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PPRV-del-L requires helper cell line. VDS or VDS-L cells were infected with PPRV-GFP or PPRV-del-L at moi = 0.01 and incubated for 48 h. Cells were fixed and stained with mouse anti-V5 tag and AlexaFluor 568-conjugated goat anti-mouse IgG. Nuclei were stained with DAPI. Confocal images were taken by sequential scanning.