Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 23;46(1):101. doi: 10.1186/s13567-015-0231-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Baron and Baron. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

PPRV-del-L behaves as heat-inactivated PPRV. VDS cells were infected with PPRV-del-L (PPRVdelL) or PPRV that had been heat-inactivated (2 h at 58 °C) (inactPPRV) at a nominal moi of 0.5. After removing unattached virus, duplicate wells were harvested immediately for RNA or cultured for 6 days (p1). After 6 days, the infected cells were subjected to 1 freeze-thaw cycle, the cell pellet harvested for RNA, and one third of the supernatant passaged to fresh cells (p2). This procedure was then repeated (p3). RT-qPCR was used to determine the relative amount of viral mRNA in each sample (A); Vero cell L13A mRNA was used as control for the recovery of RNA from each sample (B). Results are expressed as 40-(mean Ct value observed from duplicate PCRs from each of duplicate wells).