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. 2015 Sep 23;46(1):101. doi: 10.1186/s13567-015-0231-y

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© Baron and Baron. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of growth and antigenicity of PPRV-del-L and full PPRV. VDS-L cells in 6-well plates were infected with PPRV-GFP or PPRV-del-L at moi = 0.01. Duplicate wells were harvested at 0, 1, 2, 3, 4 and 5 days post infection, subjected to one freeze-thaw cycle and the released virus titrated (A). RNA was extracted from the cell pellets and viral (genome + mRNA) assayed by RT-qPCR (B). C VDS-L cells were infected with PPRV-del-L (moi = 0.01) in 175cm² flasks and cultured for 5 days. Virus antigen was prepared and used in the PPRV-H cELISA (BDSL) according to the manufacturer’s instructions. A set of samples known to be positive (% inhibition >50%) and negative (% inhibition <50%) were assayed in parallel cELISA tests using either the PPRV antigen provided with the kit or the antigen made from PPRV-del-L. All sera were assayed in duplicate in each test and the mean % inhibition obtained with each antigen plotted in the graph. The set of individual mean values for each serum were compared using a paired t-test: the mean difference was not significantly different from zero (p = 0.081). The regression line had a slope of 0.99 ± 0.02 (± S.E.) and an intercept of 1.81 ± 1.34.