Table I.
Targets for next generation sequencing assay, chromosomal location, length of the amplified regions and number of amplicons per target.
| Target | Chromosome: start-stop | Length (bp) | Amplicons |
|---|---|---|---|
| NRAS_Exon_2258071±0 | 1:115, 256, 459–115, 256, 546 | 88 | 1/1 |
| NRAS_Exon_2255597±0 | 1:115, 258, 671–115, 258, 798 | 128 | 1/1 |
| NRAS_Exon_2255265±0 | 1:115, 252, 190–115, 252, 349 | 160 | 2/2 |
| KRAS_Exon_2084598±0 | 12:25, 398, 208–25, 398, 329 | 122 | 2/2 |
| KRAS_Exon_2081588±0 | 12:25, 380, 168–25, 380, 346 | 179 | 3/3 |
| KRAS_Exon_2081272±0 | 12:25, 378, 548–25, 378, 707 | 160 | 2/2 |
| KIT_Exon_1948841±0 | 4:55, 592, 023–55, 592, 216 | 194 | 3/3 |
| KIT_Exon_1925438±0 | 4:55, 593, 582–55, 593, 708 | 127 | 2/2 |
| KIT_Exon_1923956±0 | 4:55, 594, 177-55, 594, 287 | 111 | 1/1 |
| KIT_Exon_1923106±0 | 4:55, 599, 236–55, 599, 358 | 123 | 2/2 |
| HRAS_Exon_1850745±0 | 11:533, 441–534, 375 | 935 | 11/11 |
| EGFR_Exon_2086565±0 | 7:55, 242, 415–55, 242, 513 | 99 | 1/1 |
| EGFR_Exon_2085900±0 | 7:55, 241, 614–55, 241, 736 | 123 | 2/2 |
| EGFR_Exon_2085577±0 | 7:55, 248, 986–55, 249, 171 | 186 | 3/3 |
| EGFR_Exon_2084815±0 | 7:55, 259, 412–55, 259, 567 | 156 | 3/3 |
| BRAF_Exon_2290211±0 | 7:140, 481, 376–140, 481, 493 | 118 | 2/2 |
| BRAF_Exon_2290058±0 | 7:140, 453, 075–140, 453, 193 | 119 | 1/1 |