Table II.
Comparison of Sanger sequencing, HRM and NGS methods used for mutation detection.
| Detection method | |||
|---|---|---|---|
| Parameters | Sanger sequencing | HRM | NGS |
| Limit of detection, %a | 12.50 | 5.0–7.50 | 2.0–5.0 |
| Specificity, % (true negative) | 100 (1239/1239) | 100 (1239/1239) | 100 (30/30) |
| Missed mutations, n (%) | 3/233 (1.29) | 0/233 (0.00) | 0/30 (0.00) |
| Total samples tested, n | 1472 | 1472 | 60 |
a
Frequency of mutated alleles detected in a background of wild type alleles. HRM, High-resolution melting curve; NGS, next-generation sequencing.