Table II.
Detection method | |||
---|---|---|---|
Parameters | Sanger sequencing | HRM | NGS |
Limit of detection, %a | 12.50 | 5.0–7.50 | 2.0–5.0 |
Specificity, % (true negative) | 100 (1239/1239) | 100 (1239/1239) | 100 (30/30) |
Missed mutations, n (%) | 3/233 (1.29) | 0/233 (0.00) | 0/30 (0.00) |
Total samples tested, n | 1472 | 1472 | 60 |
Frequency of mutated alleles detected in a background of wild type alleles. HRM, High-resolution melting curve; NGS, next-generation sequencing.