Fig. 1.
Fas agonist or IRBP-specific T cells promoted HMGB1 release from viable retinal cells of Wt, but not Faslpr mice. a Retina explants collected from Wt B6 mice were cultured with culture medium alone (Ctrl) or containing a cytokine mixture (IFN-γ, IL-17, TNF-α, IL-1β, and IL-6). b Retinal explants collected from Wt B6 and Faslpr mice were cultured with medium alone or containing Jo2. c Retinal explants (R) collected from Wt B6 and Faslpr mice or activated IRBP1–20-specific T cells from Wt B6 (T) were cultured alone or in combination (R + T). After 6 h culture of a–c, supernatants were assayed for HMGB1 by ELISA. **p < 0.01 compared to cells cultured with medium alone by one-way ANOVA. The results are representative of those obtained in three separate experiments. In each experiment, each control or experimental group has ≥3 individual wells with 1 retinal explant/well from different mice