Figure 4.

IF1 knockdown inhibited the NF-κB/Snai1 pathway in glioma cells. The U251 and U87 cells that were transduced with NT shRNA or IF1 shRNA were analyzed using western blotting to detect NF-κB, Snai1, E-cadherin and vimentin expression. Representative western blotting revealed that IF1 knockdown downregulated NF-κB, Snai1 and vimentin expression, but upregulated E-cadherin expression. Data are representative of multiple repeats with similar results. IF1, adenosine triphosphatase inhibitory factor 1; NT, non-targeting; shRNA, short hairpin RNA; NF-κB; nuclear factor-κB.