Figure 4.

BRG1 and BRM are not essential for exercise‐induced angiogenesis. A through C, left panels: Total distance traveled in kilometers by adult mice after 3 weeks of voluntary exercise. Data represent averages±SEM of 4 to 6 animals per genotype. A through C, middle panels: Representative images of stained quadriceps from sedentary and exercised control and SWI/SNF mutant mice. Quadriceps were stained with PECAM‐1 (red) to visualize blood vessels and laminin (green) to outline muscle fibers (insert). Scale bars=50 μm. A through C, right panels: Quantification of immunostaining experiments represented at left. Tissue from each animal was stained 3 separate times, and vessels and muscle fibers from 10 fields were counted for each staining experiment. Data represent averages±SEM of blood vessels per muscle fiber from 4 to 6 animals, normalized to sedentary controls. *P<0.05; Student t test. Brg1 indicates Brahma‐related gene 1; Brm, Brahma; N.S., not significant; PECAM‐1, platelet endothelial cell adhesion molecule 1; SWI/SNF, SWItch/Sucrose NonFermentable.